生物技术通报2024，Vol.40Issue(11)：58-67,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0677
杨树桑黄多糖提取条件优化及其抑菌活性研究
Study on the Optimization of Extraction Conditions and Antibacterial Activities of Polysaccharides from Sanghuongporus vaninii
摘要
Abstract
[Objective]The aim is to optimize the extraction process of Sanghuongporus vaninii polysaccharides(SVP),explore the antibacterial mechanism of SVP,and provide reference for the development of natural antimicrobial agents.[Method]Based on single factor experiments and response surface analysis,we optimized the process of extracting polysaccharides by water extraction and alcohol precipitation,and improve the polysaccharides extraction yield.By measuring the minimum inhibitory concentration,growth curve,and enzyme activities,we studied the antibacterial activity of SVP against Escherichia coli and Staphylococcus aureus.[Result]When the extraction temperature is 86℃,the time is 1.6 h,and the ratio of material to liquid is 1:24 g/mL,the extraction yield of polysaccharides is 4.07%.The minimum inhibitory concentrations of polysaccharides against E.coli and S.aureus are 2.000 mg/mL and 1.500 mg/mL,respectively.Compared with the negative control group,the extracellular alkaline phosphatase(AKP)activity of E.coli and S.aureus increased by 3.7 times(P＜0.05)and 2.6 times(P＜0.05),the enzyme activity of β-galactosidase increased by 6.2%(P＜0.01)and 7.3%(P＜0.01),the ΔOD 260nm increased by 60%(P＜0.01)and 1.2 times(P＜0.01),the intracellular Na+/K+-ATPase activity decreased by 28.9%(P＜0.001)and 34.8%(P＜0.001),the Ca2+-ATPase activity decreased by 43.2%(P＜0.001)and 35.6%(P＜0.001),the ATPase activity decreased by 20.1%(P＜0.001)and 34.6%(P＜0.001),the malate dehydrogenase(MDH)activity decreased by 23.5%(P＜0.001)and 28.7%(P＜0.001),and the succinate dehydrogenase(SDH)activity decreased by 17.9%(P＜0.01)and 13.8%(P＜0.01),respectively.[Conclusion]After response surface optimization,the actual extraction yield of SVP is 5.71%higher than the predicted extraction yield,and SVP has a strong inhibitory effect on E.coli and S.aureus.Its mechanism of action may be related to the destruction of bacterial cell membranes,inhibition of energy metabolism enzymes,etc.关键词
真菌/杨树桑黄多糖/响应面/抑菌活性Key words
fungus/Sanghuongporus vaninii polysaccharides/response surface/antibacterial activity引用本文复制引用
叶卓妍,周家淇,林丹媛,孙赫男,王艳珍..杨树桑黄多糖提取条件优化及其抑菌活性研究[J].生物技术通报,2024,40(11):58-67,10.基金项目
珠海科技学院"三个层次"人才建设工程,广东省普通高校天然产物功能成分利用工程技术研究中心(2022GCZX012),广东省"大学生创新创业训练计划"项目(S202213684026,S202413684026) （2022GCZX012）
