生物技术通报2024，Vol.40Issue(11)：78-87,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0985
珊瑚状猴头菌多糖超声辅助提取工艺优化及抗结直肠癌活性研究
Study on the Optimization of Ultrasonic-assisted Extraction Process of Hericium coralloides(Scop.)Pers.Polysaccharides and Its Anti-colorectal Cancer Activity
摘要
Abstract
[Objective]Hericium coralloides(Scop.)Pers.is a medicinal and edible fungus.This study focused on optimizing the ultrasonic extraction process of its polysaccharides(HCP)and exploring the in vitro anti-colorectal cancer activity and underlying mechanisms of HCP.[Method]Single-factor experiments combined with response surface methodology(RSM)S was used to optimize the ultrasonic extraction conditions of HCP.MTT assays and Western blot analyses were applied to investigate the anti-colorectal cancer activity and mechanisms of HCP.[Result]The optimal ultrasonic extraction conditions for H.coralloides polysaccharides were as follows:extraction power of 417 W,liquid-to-material ratio of 62 mL/g,and extraction time of 52.5 min.The predicted extraction yield of polysaccharides was 19.61%,with an actual yield of(19.58±0.05)%.HCP inhibited the growth of SW480 cells by reducing the phosphorylation levels of IKK,IκBα,and NF-κB,thereby decreasing the secretion of the pro-inflammatory factor IL-6.The half-maximal inhibitory concentration(IC50)was 1.246 mg/mL.[Conclusion]The optimal ultrasonic-assisted extraction rate of H.coralloides polysaccharides was 19.58%.HCP suppressed the secretion of pro-inflammatory cytokines by inhibiting the NF-κB signaling pathway,thus inhibiting the growth of SW480 cells.These findings suggest that HCP has potential therapeutic effects on colorectal cancer.关键词
珊瑚状猴头菌/抗结直肠癌活性/超声提取/响应面法/多糖/NF-κB信号通路Key words
Hericium coralloides/anti-colorectal cancer activity/ultrasonic extraction/response surface optimization/polysaccharides/NF-κB signaling pathway引用本文复制引用
余金岂,邬柯奕,陈正睿,王婧涵,张鑫,王春月..珊瑚状猴头菌多糖超声辅助提取工艺优化及抗结直肠癌活性研究[J].生物技术通报,2024,40(11):78-87,10.基金项目
国家食用菌产业技术体系(CARS-20),国家级大学生科技创新训练计划(202310193047),天津市合成生物技术创新能力提升行动项目(TSBICIP-CXRC-006) （CARS-20）
