生物技术通报2024，Vol.40Issue(11)：125-141,17.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0360
基于质谱的单细胞蛋白质组学技术的发展及应用
Development and Application of Mass Spectrometry-based Single-cell Proteomics Technologies
摘要
Abstract
Single-cell multi-omics technologies have brought new vitality into life sciences and medicine and enable us to explore the unknowns of life and expand the boundaries of medicine from a more microscopic cellular scale,a higher-dimensional spatial-temporal perspective,and a more intersectional vision of genomics.Single-cell proteomics(SCP)is the study of the temporal and spatial heterogeneity of protein expression in a single or a single type of cell.Despite technical difficulties such as throughput,sensitivity,and resolution,mass spectrometry-based cell-scale spatiotemporal proteomics technologies have attracted much attention due to its characteristics of unbiased detection,multiple identification types and accurate quantification.This paper reviews the development of single-cell proteomics in recent years,introduces the related technological advances in four aspects,namely,single-cell sorting,sample pre-treatment,mass spectrometry detection,and data analysis,and discusses its applications and prospects for development in life science and medical research.Spatiotemporal proteomics research at the single-cell level presents both challenges and opportunities,and the emerging single-cell proteomics technologies will undoubtedly provide new ideas and methods,cognitive clues,and multimodal data,and other valuable resources for related research fields.关键词
单细胞蛋白质组学/质谱/细胞尺度的时空蛋白质组学技术Key words
single-cell proteomics(SCP)/mass spectrometry(MS)/cell-scale spatiotemporal proteomics technology引用本文复制引用
顾蕾,张誉露,唐尚睿,于浩月,李辰..基于质谱的单细胞蛋白质组学技术的发展及应用[J].生物技术通报,2024,40(11):125-141,17.基金项目
国家自然科学基金面上项目(82373446),上海市"科技创新行动计划"自然科学基金面上项目(23ZR1435800),上海市"科技创新行动计划"技术标准项目(21DZ2201700) （82373446）
