生物技术通报2024，Vol.40Issue(11)：142-151,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0297
基于SCoT分子标记分析白术种质资源遗传多样性及DNA指纹图谱构建
Genetic Diversity Analysis and DNA Fingerprint Construction of Atractylodes macrocephala Germplasm Resources Based on SCoT Molecular Markers
摘要
Abstract
[Objective]Using SCoT molecular marker technology,the genetic diversity analysis and DNA fingerprinting were conducted on 17 germplasm resources of Atractylodes macrocephala,providing a theoretical basis for the identification,preservation,and breeding of new varieties of A.macrocephala germplasm.[Method]Thirteen core primers were selected from 92 SCoT primers for molecular labeling of A.macrocephala.Popgene1.32 software and NTSYS pc 2.10e software were used for diversity analysis and cluster analysis of A.macrocephala germplasm resources.A DNA molecular fingerprint map of A.macrocephala was constructed by combining the core primers of SCoT-9,SCoT-12,and SCoT-41.[Result]A total of 192 bands were obtained from 17 samples of A.macrocephala amplified by 13 SCoT core primers,including 165 polymorphic bands,with an average percentage of 85.94%.The genetic similarity coefficient(GS)and genetic distance(GD)ranged from 0.578 1 to 0.875 0 and 0.133 5 to 0.548 0,respectively.The observed number of alleles(Na)in the A.macrocephala germplasm was 1.859 4,the effective number of alleles(Ne)was 1.418 0,the Nei's genetic diversity index(He)was 0.256 3,and the Shannon information index(I)was 0.396 8.Cluster analysis showed that A.macrocephala was a cultivated variety,while the wild variety of Daweishan was separately clustered into another category.By the 17 constructed DNA fingerprint maps of A.macrocephala germplasm resources,the samples were distinguished and accurately identified.[Conclusion]The germplasm resources of A.macrocephala have relatively rich genetic diversity,but the genetic differences among cultivated A.macrocephala from different regions are low,indicating that geographical location is not a decisive factor in determining the distance of A.macrocephala kinship.关键词
白术/SCoT分子标记/遗传多样性分析/DNA指纹图谱Key words
Atractylodes macrocephala/SCoT molecular markers/genetic diversity analysis/DNA fingerprinting引用本文复制引用
李晴,石雨荷,朱珏,李晓玲,侯超文,童巧珍..基于SCoT分子标记分析白术种质资源遗传多样性及DNA指纹图谱构建[J].生物技术通报,2024,40(11):142-151,10.基金项目
国家中药材产业技术体系食用百合龙山综合试验站(CARS-21),湖南省科技厅科技特派员服务乡村振兴项目(2021NK4240),湖南省教育厅重点项目(22A0272),湖南中医药大学研究生创新项目(2021CX51) （CARS-21）
