生物技术通报2024，Vol.40Issue(11)：152-161,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0405
水稻OsRhoGDI1过表达影响粒型及种子活力
Overexpression of OsRhoGDI1 Gene Regulates Grain Shape and Seed Vigor in Rice
摘要
Abstract
[Objective]OsRhoGDI1,a rice RhoGDI gene,was isolated from young panicles through yeast two-hybrid screening,using Rho/Rop protein OsRac5 as bait.RhoGDIs genes encode Rho GDP dissociation inhibitors,which negatively regulate the activities of Rho proteins.Transgenic technology was applied to identify the functions of OsRhoGDI1,aiming to lay a foundation for further research on the functional relationship between OsRhoGDI1 and OsRac5 in rice growth and development.[Method]OsRhoGDI1-overexpressed vector was constructed,and rice were transformed to obtain positive transgenic plants.The grain shape and germination characteristics of transgenic rice was analyzed.The expressions of grain-shape-regulating genes in grain development were detected through RT-qPCR,and scanning electron microscopy was used to detect the characteristics of glume cells.The germination characteristics were compared between OsRhoGDI1-overexpressed rice and control,and the expressions of the genes involved in synthesis and metabolism of gibberellin(GA)and abscisic acid(ABA)were detected by RT-qPCR,as well as the expressions and activities of α-amylases.[Result]Compared with the control rice,the OsRhoGDI1-overexpressed rice showed a significant increase in grain length,decrease in grain width,thickness and thousand grain weight.The expressions of grain-shape-regulating gene GS2,GS5,GS6 and GLW7 in young panicles also reduced to varying degrees,while the expression of GW2 increased.The lengths of glume cells in the transgenic rice increased,while the width decreased.The germination of transgenic rice was earlier than that of control,and the expressions of GA synthesis genes,ABA catabolic genes,and α-amylase genes increased to varying degrees,while the expressions of GA catabolic genes and ABA synthesis genes decreased.α-amylase activities were also enhanced in the transgenic rice,compared to the control.[Conclusion]The overexpression of OsRhoGDI1 gene regulates rice grain shape by altering the expressions of grain shape genes,leading to elongation of glume epidermal cells,resulting in increase of grain length and decrease of grain width.Meanwhile,the overexpression of this gene also changes the expressions of germination-related genes,increase the activities of α-amylase,thus enhance seed vigor and promote germination.关键词
水稻/OsRhoGDI1/粒型/萌发/激素/α-淀粉酶Key words
rice/OsRhoGDI1/grain shape/germination/hormones/α-amylase引用本文复制引用
戚旺,梁卫红,毕一凡,轩强兵,张新,周慧岗,聂圆情,程彪彪,张禹舜,王俊杰..水稻OsRhoGDI1过表达影响粒型及种子活力[J].生物技术通报,2024,40(11):152-161,10.基金项目
国家自然科学基金项目(32201248),河南省科技研发计划联合基金(222301420106),河南省重点研发与推广专项(242102111164),河南省自然科学基金青年基金项目(222300420202),河南省高等学校重点科研项目(23A180020) （32201248）
