生物技术通报2024，Vol.40Issue(11)：162-168,7.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0308
番茄YABBY2b功能鉴定及下游基因的表达分析
Functional Identification of YABBY2b Gene and Expression Analysis of Downstream Genes in Tomato
摘要
Abstract
[Objective]To investigate the function of tomato SlYABBY2b and elucidate the mechanism of fruit size regulation,thus laying the foundation for enhancing the gene networks involved in fruit development and uncovering valuable tomato(Solanum lycopersicum L.)gene resources in China.[Method]Using CRISPR/Cas9 technology,yabby2b mutant lines were generated by editing the SlYABBY2b gene in tomato'Ailsa Craig'.The phenotypic changes of the yabby2b mutants were analyzed,followed by quantification of fruit cell numbers through paraffin sectioning.Expression changes of candidate genes in the yabby2b mutants were assessed using RT-qPCR,along with the analysis of promoter regulatory elements of these genes.[Result]Chimeric or biallelic mutants were identified in the T0 generation,while three homozygous mutants were isolated in the T1 generation,devoid of transgenic elements.Phenotypic observations revealed that yabby2b mutant plants demonstrated the reduced height and smaller fruits compared to the wild type.Further investigation into fruit development indicated that the ovary of yabby2b mutants was smaller with decreased ovary cell numbers during early fruit development.Analysis of candidate gene expressions revealed an up-regulation of SlFW2.2,a cell division suppressor,in response to the SlYABBY2b mutation,along with the presence of regulatory elements of YABBY transcription factors on the SlFW2.2 promoter.[Conclusion]These findings suggest that SlYABBY2b may play a role in regulating plant height and fruit size in tomatoes by modulating SlFW2.2 gene expression,thereby influencing cell division in early fruit development and ultimately determining fruit size.关键词
番茄/SlYABBY2b/果实大小/细胞分裂/SlFW2.2Key words
tomato/SlYABBY2b/fruit size/cell division/SlFW2.2引用本文复制引用
孙美华,孙慧贤,田林林,苗妍秀,侯雷平,齐明芳,李天来..番茄YABBY2b功能鉴定及下游基因的表达分析[J].生物技术通报,2024,40(11):162-168,7.基金项目
山西省基础研究计划(202103021223171,20210302124242),山西农业大学博士科研启动项目(2021BQ31),山西省博士来晋工作奖励(SXBYKY2021071) （202103021223171,20210302124242）
