生物技术通报2024，Vol.40Issue(11)：184-191,8.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0328
枣ABF/AREB基因家族鉴定及其在果实发育中的表达分析
Identification of ABF/AREB Gene Family and Their Expression Analysis in Jujube Fruit
摘要
Abstract
[Objective]ABFs(ABRE binding factors)/AREB(ABA responsive element binding)transcription factors,which not only participate in the plant stress response,but also affect the development of plants and fruits.Understanding its role in jujube fruit development provides a basis for further study of jujube ABF gene function.[Method]We conducted genome-wide identification of jujube ABF gene and analyzed the physicochemical analysis,subcellular localization,gene structure,motifs,phylogenetic relationships,promoter cis-acting elements and expression patterns.[Result]Five ABF genes were found in the jujube genome and located on five different pseudochromosomes.The length of the coding amino acid sequence of ZjABF was 321 to 474 aa,the isoelectric point was 7.75 to 9.85,the hydrophilic coefficient was between-0.807 and-0.592,and all five members were hydrophilic proteins.The instability coefficient ranged from 35.62 to 61.51.Gene structure and motif analysis showed that all ABF family members had UTR,containing 3 to 5 exons and 6 to 7 motifs.Phylogenetic trees grouped the ABF family into three large classes.Promoter cis-acting element analysis showed that jujube ABF gene family promoters had multiple cis-acting elements related to response hormones and abiotic stress.By analyzing the transcriptome data at different stages of fruit development,all ZjABFs except ZjABF4 were differently expressed,indicating that the ABF family played a regulatory role in fruit development.Interacting proteins of ZjABF3,ZjABF4,and ZjABF5 were predicted using homologous proteins.[Conclusion]The structure of ABF family in jujube is conserved,and there are multiple cis-acting elements in the promoter region that respond to hormones and abiotic stress,with different expression patterns during different stages of fruit development.关键词
枣/ABF/AREB基因家族/生物信息学分析/果实发育Key words
Ziziphus jujuba Mill./ABF/AREB gene family/bioinformatics analysis/fruit development引用本文复制引用
杨冲,程莎莎,艾长丰,赵璇,刘孟军..枣ABF/AREB基因家族鉴定及其在果实发育中的表达分析[J].生物技术通报,2024,40(11):184-191,8.基金项目
国家自然科学基金项目(32101542),河北省自然科学基金项目(C2021204101),河北农业大学引进人才科研专项基金(3002054) （32101542）
