生物技术通报2024，Vol.40Issue(11)：227-235,9.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0318
狗尾草ARF基因家族的鉴定与表达分析
Identification and Expression Analysis of ARF Gene Family in Setaria viridis
摘要
Abstract
[Objective]The auxin response factor(ARF)plays an important role in plant growth and development through regulating the expression of auxin response genes.The SvARF gene family members were identified from the Setaria viridis genome,and the expression characteristics of related genes were analyzed,providing support for further research on the relationship between SvARF and the growth and de-velopment of S.viridis.[Method]The ARF transcription factors were identified from the S.viridis genome by bioinformatics.The phylogenetic evolution,chromosome location,and gene structure were also analyzed.Real-time quantitative polymerase chain reaction(RT-qPCR)were used to analyze the expression responses of SvARF gene to 2,4-D isooctyl esters.[Result]A total of 24 SvARF genes were identified.The mem-bers of SvARF gene family can be divided into 4 subfamilies.SvARF genes in the same subfamily shared similar expression pattern and genes in subfamily Ⅲ expressed specifically in the tillers.After 2,4-D isooctyl ester treatment,the relative expressions of nine SvARF genes changed significantly.SvARF5,SvARF6,SvARF9,SvARF10,SvARF17 and SvARF18 were up-regulated,while SvARF14,SvARF22,and SvARF24 were down-regulated.[Conclusion]There are 24 members in the SvARF gene family.Auxin pesticide affects the expressions of SvARF genes,which may decrease the plant tiller number.关键词
ARF转录因子/狗尾草/系统进化/结构特征/表达分析/分蘖/2,4-D异辛酯Key words
ARF transcription factor/Setaria viridis/phylogenetic evolution/structure characteristics/gene expression/tillering/2,4-D isooctyl ester引用本文复制引用
王其昕,张凌馨,钟妮娜,熊王丹..狗尾草ARF基因家族的鉴定与表达分析[J].生物技术通报,2024,40(11):227-235,9.基金项目
国家自然科学基金项目(31872879),山东省自然科学基金项目(ZR2023MC071),山东省大学生创新创业项目(S202310435231) （31872879）
