生物技术通报2024，Vol.40Issue(11)：296-311,16.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0150
不同盐度培养下海洋真菌Aspergillus unguis DLEP2008001生物活性及次生代谢组变化
Variation of Bioactivities and Secondary Metabolomics of Marine Fungus Aspergillus unguis DLEP2008001 Cultured under Different Salinities
摘要
Abstract
[Objective]A marine fungus Aspergillus unguis DLEP2008001 was used for investigating the influence of salinity on the bioactivity and secondary metabolites.[Method]The crude extract samples of this strain cultured under different salinities in solid and liquid mediums for 14 and 28 days were analyzed by thin layer chromatography and antioxidative,acetylcholinesterase(AChE)inhibitory,and antimicrobial bioautographies to compare the bioactive secondary metabolites.The secondary metabolite diversity was further investigated by high performance liquid chromatography and"Multi-approach Assisted Feature Based Molecular Network(FBMN)"metabolomic analytical method based on liquid chromatography-tandem mass spectrometry(LC-MS/MS).[Result]The salinity regulated the yield and diversity of antioxidant,AChE inhibitory ingredients and anti-microbial.In seawater-potato-sucrose liquid medium,the strain presented the most fertile feature metabolites when cultured for 28 d under salinity of 35 g/L,while in rice solid medium,it produced the most abundant predominant compounds under salinity of 5 g/L and 35 g/L as the second best condition.The average molecular weights of the predominant metabolites from solid culture generally deceased with the ascending of the salinity and they were mostly small molecules with multiple hydroxyl and amino groups,which are speculated to involve the adjustment of osmotic pressure.[Conclusion]Salinity exerts a significant influence on the bioactivity and secondary metabolites of this strain.关键词
不同盐度/海洋真菌/分子网络/次生代谢产物/生物活性Key words
different salinities/marine fungi/molecular network/secondary metabolites/biological activity引用本文复制引用
康晓博,张璟汐,卢甜甜,刘亚月,周龙建,张翼..不同盐度培养下海洋真菌Aspergillus unguis DLEP2008001生物活性及次生代谢组变化[J].生物技术通报,2024,40(11):296-311,16.基金项目
广东省基础与应用基础研究基金自然科学基金(面上项目)(2022A1515010783),广东省普通高校重点领域专项(生物医药与健康)(2021ZDZX2064),深圳市科创委基础研究面上项目(JCYJ20220530162014032),湛江市海洋青年人才创新项目(2022E05010),广东海洋大学大学生创新创业训练计划项目(S202110566014),广东海洋大学研究生教育创新计划项目(202232) （面上项目）
