土壤学报2024，Vol.61Issue(6)：1703-1713,11.DOI:10.11766/trxb202306130230
稻田土壤中病毒多样性研究
Virus Diversity Studies in Paddy Soils
摘要
Abstract
[Objective]Viruses are the most abundant and diverse group of microorganisms on Earth as they can be found in a wide range of organisms and environments.Despite their ubiquity,there are relatively few studies on the virome in paddy soil systems,which is an area that warrants investigation.[Method]In this study,virus sequences were mined from rice soil macrogenome sequencing data in public databases and characterized.[Result]A database of paddy soil viruses(PSVD)was constructed,which contained 8 791 viral operational taxonomic units(vOTUs)and 168 940 protein sequences.In PSVD,31.41%of the sequences could predict the taxonomic status of the species.These viral sequences belonged to 76 known virus families,with the majority of viruses coming from the Caudoviricetes,and their hosts were mainly distributed across 11 bacterial phyla.Also,Auxiliary Metabolic Genes(AMGs)carried by rice field soil viruses were analyzed,and 39 carbohydrate-active enzymes(CAZymes)genes,which may have been widely distributed in rice soil systems,were identified,and the presence of these genes contributed to biogeochemical cycling.[Conclusion]In summary,this study innovatively constructed a PSVD database(https://github.com/lzk98/PSVD)and used DRAM-v to mine AMG genes in paddy soil systems,thus,laying a research foundation for later studies of paddy soil virus groups and providing new insights for the resolution of paddy virus communities.关键词
病毒组学/土壤/稻田/碳水化合物活性酶Key words
Viromic/Soil/Rice field/Carbohydrate-active enzymes分类
农业科技引用本文复制引用
刘泽锟,黄士轩,杨美艳..稻田土壤中病毒多样性研究[J].土壤学报,2024,61(6):1703-1713,11.基金项目
国家自然科学基金项目(32202194)、广东省微生物安全与健康重点实验室开放课题(2020B121201009-PKLMSH003-2021)和广州市重点研发计划项目(SL2022B03J01243)资助 Supported by the National Natural Science Foundation of China(No.32202194),the Guangdong Provincial Key Laboratory of Microbial Safety and Health Open Topic(No.2020B121201009-PKLMSH003-2021),and the Key R&D Projects of Guangzhou(No.SL2022B03J01243) （32202194）
