西南林业大学学报2024，Vol.44Issue(11)：184-193,10.DOI:10.11929/j.swfu.202401061
诱导山苍子胚性愈伤组织的影响因素研究
The Factors Influencing the Embryogenic Callus Induction of Litsea cubeba
摘要
Abstract
In order to screen out the best induction conditions for embryogenic calls formation of Litsea cubeba,nearly mature seed embryos of L.cubeba good single plants were used as explants to analyze the effects of different disinfection methods,hormone combinations,carbon source type and seed source type on the embryo-genic calls induction.The results showed that sterilizing the endocarp with 75％ethanol for 5 min and rinsing with sterile water for 5 times resulted in the lowest contamination rate of 0.00％;Nanchang City,Jiangxi Province,was the best seed source location for embryogenic calls induction,with an induction rate of 7.04％;and the addition of the exogenous hormone 1.0 mg/L 6-BA+0.2 mg/L 2,4-D+0.5 mg/L IAA had the highest rate of embryogenic calls,with a total induction rate of 17.50％.In this study,we successfully induced embryogenic calls of L.cubeba and achieved proliferation of embryogenic calls and differentiation of somatic embryos in medium MS+0.5 mg/L 6-BA+0.1 mg/L 2,4-D+0.25 mg/L IAA,and then realized the germination of somatic embryos.关键词
山苍子/体细胞胚胎/愈伤组织/激素/增殖Key words
Litsea cubeba/somatic embryogenesis/embryogenic callus/hormone/proliferation分类
农业科技引用本文复制引用
毛卓佳,汪加魏,易敏,蒋润迪,陈尚钘,梁擎宇,吴祖明,王宗德,范国荣,付丹..诱导山苍子胚性愈伤组织的影响因素研究[J].西南林业大学学报,2024,44(11):184-193,10.基金项目
江西省林业局樟树研究专项(2020CXZX07-2020-01-03)资助 （2020CXZX07-2020-01-03）
国家重点研发计划项目(2017YFD0601303)资助 （2017YFD0601303）
江西省重大科技研发专项(20203ABC28W016)资助 （20203ABC28W016）
江西省级大学生创新创业训练计划项目(S202210410045)资助. （S202210410045）
