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基于LC-MS的樟叶越桔愈伤组织次生代谢成分分析

王玮 李楚然 武波晓 刘云 杨晓琴 朱国磊 赵平

西南林业大学学报2024,Vol.44Issue(11):194-199,6.
西南林业大学学报2024,Vol.44Issue(11):194-199,6.DOI:10.11929/j.swfu.202308004

基于LC-MS的樟叶越桔愈伤组织次生代谢成分分析

LC-MS Analysis of Secondary Metabolites in the Callus of Vaccinium dunalianum

王玮 1李楚然 1武波晓 1刘云 2杨晓琴 1朱国磊 1赵平3

作者信息

  • 1. 西南林业大学西南地区林业生物质资源高效利用国家林业和草原局重点实验室,云南 昆明 650233
  • 2. 西南林业大学西南山地森林资源保育与利用教育部重点实验室,云南 昆明 650233
  • 3. 西南林业大学西南地区林业生物质资源高效利用国家林业和草原局重点实验室,云南 昆明 650233||西南林业大学西南山地森林资源保育与利用教育部重点实验室,云南 昆明 650233
  • 折叠

摘要

Abstract

The investigation focused on the healthy callus of Vaccinium dunalianum,and the methanol con-tent was analyzed using LC-MS technology.The results showed that a total of 281 compounds divided into 14 categories were detected in the positive ion mode,which mainly including 58 terpenoids,41 alkaloids,28 organic acids,24 heterocyclic compounds,22 glycosides and 13 alcohols,with a relative content of 16.866%,13.711%,6.108%,10.138%,12.488%and 17.513%,respectively.A total of 71 compounds divided into 13 categories were detected in the negative ion mode,among which 14 alkaloids,9 organic acids,9 glycosides,7 terpenoids and 7 es-ters were the main components with a relative content of 5.839%,72.653%,3.423%,2.062%and 2.735%,respect-ively.Especially,the relative content of pyruvic acid was the highest at 47.474%.The results will provide a basis for the further regulation of the major chemical constituents in the callus of V dunalianum.

关键词

樟叶越桔/愈伤组织/化学成分/次生代谢/LC-MS

Key words

Vaccinium dunalianum/callus/chemical constituent/secondary metabolism/LC-MS

分类

化学化工

引用本文复制引用

王玮,李楚然,武波晓,刘云,杨晓琴,朱国磊,赵平..基于LC-MS的樟叶越桔愈伤组织次生代谢成分分析[J].西南林业大学学报,2024,44(11):194-199,6.

基金项目

国家自然科学基金项目(32060107,32060327)资助. (32060107,32060327)

西南林业大学学报

OA北大核心CSTPCD

2095-1914

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