针刺研究2024，Vol.49Issue(12)：1231-1238,8.DOI:10.13702/j.1000-0607.20240671
电针"足三里"改善慢性心力衰竭小鼠心功能的效应机制研究
Mechanism of electroacupuncture at"Zusanli"(ST36)in improving cardiac function in mice with chronic heart failure
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)at"Zusanli"(ST36)on improving cardiac function in mice with chronic heart failure(CHF),so as to explore the mechanism of its regulation on the autonomic nervous system.Methods Eighteen C57BL/6J mice were randomly divided into sham-surgery,model,and EA groups,with 6 mice in each group.The model of myocardial ischemia followed by CHF was induced by ligation of the anterior descending branch of the left coronary artery.Two weeks after modeling surgery,EA intervention(0.5 mA,2 Hz/15 Hz)was performed bilaterally at ST36 in the EA group,20 min each time,once every other day for 4 weeks.The left ventricular ejection fraction(LVEF),left ventricular fractional shortening(LVFS),left ventricular end-systolic diameter(LVESD),left ventricular end-systolic volume(LVESV),left ventricular end-diastolic diameter(LVEDD),and left ventricular end-diastolic volume(LVEDV)were evaluated using echocardiographic imaging.The exercise tolerance was observed in an exhaustive test.Electrocardiograms were recorded using an electrophysiological recording system to analyze heart rate(HR)changes before and after EA,and frequency domain analysis was used to analyze the heart rate variability(HRV),including low-frequency(LF)component,high-frequency(HF)component,and their ratio(LF/HF).Immunofluorescence was used to observe the number of choline acetyltransferase(ChAT)+/c-fos+co-labeled neurons in the dorsal motor nucleus of the vagus(DMV).Results In terms of cardiac function,compared with the sham-surgery group,the LVEF and LVFS were decreased(P<0.001)and the LVESD,LVEDD,LVESV,and LVEDV were increased(P<0.001,P<0.01)two weeks after modeling of mice in the model group.After 2 weeks of EA intervention,the LVESD and LVESV in the EA group were significantly smaller than those in the model group(P<0.05).After 4 weeks of intervention,the LVEF and LVFS in the EA group were significantly higher than those in the model group(P<0.05),and the LVESD and LVESV were significantly reduced(P<0.05,P<0.01).Compared with the model group,EA significantly improved the exercise tolerance of CHF mice(P<0.05).Compared with the sham-surgery group,the LF/HF in the model group and EA group were significantly increased(P<0.05)2 weeks after modeling,indicating autonomic imbalance.After 4 weeks of intervention,the LF/HF in the EA group showed lower trend than that in the model group but the difference was not statistically significant.Additionally,after immediate EA,HR decreased(P<0.05,P<0.01),HF increased(P<0.01),and both LF and LF/HF decreased significantly(P<0.05)of mice in the EA group,indicating that EA corrected the autonomic imbalance in CHF mice.The number of ChAT+/c-fos+co-labeled neurons in the DMV of the EA group was significantly higher(P<0.001)than that in the model group.Conclusion EA at ST36 significantly improved cardiac function and exercise tolerance in CHF mice.The underlying mechanism may be related to the activation of vagus efferent nerve and the regulation of autonomic nerve balance.关键词
电针/足三里/迷走神经/慢性心力衰竭Key words
Electroacupuncture/"Zusanli"(ST36)/Vagus nerve/Chronic heart failure引用本文复制引用
王莉莉,万红叶,邢敏,宿杨帅,张知云,刘筱羽,景向红..电针"足三里"改善慢性心力衰竭小鼠心功能的效应机制研究[J].针刺研究,2024,49(12):1231-1238,8.基金项目
国家重点研发计划项目(No.2022YFC3500702) （No.2022YFC3500702）
国家自然科学基金面上项目(No.82374284) （No.82374284）
针药结合教育部重点实验室(南京中医药大学)开放课题(No.zykf202306) （南京中医药大学）
中央级公益性科研院所基本科研业务费专项(No.ZZ-YQ2023001) （No.ZZ-YQ2023001）
