Peptide Cy_(RL-QN15)accelerates hair regeneration in diabetic mice by binding to the Frizzled-7 receptorOACSTPCD
Individuals with diabetes frequently face serious challenges,including delayed wound healing and increased risk of infection.Notably,the regeneration of hair follicles plays a crucial role in accelerating diabetic skin damage repair,reducing the risk of infection,and enhancing overall skin health.Research has predominantly emphasized the re-epithelialization of diabetic wounds,with a paucity of in-depth studies on hair follicle regeneration.In the current study,we explored the effects of a bioactive amphibian-derived peptide,Cy_(RL-QN15),on promoting hair regeneration in a diabetic skin model.In vivo experiments demonstrated that local treatment with Cy_(RL-QN15)not only accelerated wound healing of scalded skin on the backs of diabetic Kunming(KM)mice but also improved growth of damaged hair follicles.Additionally,back-shaved diabetic C57BL/6 mice showed a significant increase in the growth of newly formed hair after 28 days of continuous Cy_(RL-QN15)treatment.Further analysis indicated that the hairregenerating effects of Cy_(RL-QN15)were closely associated with the proliferation and migration of hair follicle stem cells(HFSCs).Cy_(RL-QN15)enhanced intracellularβ-catenin expression by binding to the Frizzled-7 receptor on the surface of HFSCs.The up-regulation inβ-catenin modulated the levels of downstream proteins,such as cMYC,Cyclin D1,and Lef1,ultimately inducing hair regeneration.This study not only reveals the robust effects of the bioactive peptide Cy_(RL-QN15)in hair follicle regeneration but also provides novel avenues for the development of more targeted and effective therapeutics for diabetic wound healing in the future.
Yu-Tong Wu;Ze-Qiong Ru;Ying Peng;Zhe Fu;Qiu-Ye Jia;Zi-Jian Kang;Yuan-Sheng Li;Yu-Bing Huang;Sai-Ge Yin;Kun Guo;Nai-Xin Liu;Cheng-An Feng;Jing Tang;Bai-Yu Zhang;Zhi Yang;Ying Wang;Xin-Wang Yang
Department of Anatomy and Histology&Embryology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,ChinaDepartment of Anatomy and Histology&Embryology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,ChinaDepartment of Anatomy and Histology&Embryology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,ChinaDepartment of Anatomy and Histology&Embryology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,ChinaDepartment of Anatomy and Histology&Embryology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,ChinaDepartment of Anatomy and Histology&Embryology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,ChinaDepartment of Anatomy and Histology&Embryology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,ChinaDepartment of Biochemistry and Molecular Biology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,ChinaDepartment of Anatomy and Histology&Embryology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,ChinaDepartment of Anatomy and Histology&Embryology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,ChinaDepartment of Anatomy and Histology&Embryology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,ChinaDepartment of Anatomy and Histology&Embryology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,ChinaDepartment of Biochemistry and Molecular Biology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,ChinaDepartment of Urology,First Affiliated Hospital of Kunming Medical University,Kunming,Yunnan 650500,ChinaDermatology,First Affiliated Hospital of Kunming Medical University,Kunming,Yunnan 650500,ChinaKey Laboratory of Chemistry in Ethnic Medicinal Resources&Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal Endophytes,State Ethnic Affairs Commission&Ministry of Education,School of Ethnic Medicine,Yunnan Minzu University,Kunming,Yunnan 650504,ChinaDepartment of Anatomy and Histology&Embryology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,China Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming,Yunnan 650500,China
临床医学
CyRL-QN15Hair follicle stem cells(HFSCs)β-cateninFrizzled-7
《Zoological Research》 2024 (6)
P.1287-1299,13
supported by the Key Program of Yunnan Applied Basic Research Project(202301AS070036)Outstanding Youth Program of Yunnan Applied Basic Research Project-Kunming Medical University Union Foundation(202301AY070001-301)First-Class Discipline Team of Skin&Mucosal Regenerative Medicine of Kunming Medical University(2024XKTDTS10)National Natural Science Foundation of China(32360138,32301054,32060212,82371567)Program of Yunnan Applied Basic Research Project-Kunming Medical University Union Foundation(202301AY070001-012,202101AY070001-006,202301AY070001-198,202101AY070001-035,202101AY070001-036,202401AY070001-028,202401AY070001-068)。
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