北京中医药大学学报2024，Vol.47Issue(12)：1693-1701,9.DOI:10.3969/j.issn.1006-2157.2024.12.009
活血解毒方对干燥综合征小鼠颌下腺细胞凋亡的影响研究
Study on the effect of Huoxue Jiedu Formula on apoptosis of submaxillary gland cells in mice with Sjögren syndrome
摘要
Abstract
Objective To explore the effects of Huoxue Jiedu Formula on the expression of the apoptosis-related factors B-cell lymphoma-2(Bcl-2)and survivin in submaxillary gland cells in mice with Sjögren syndrome(SS).Methods Using 15 healthy C57BL/6J female mice as the blank control group.Seventy-five of non-obese diabetes mellitus female mice were classified as SS model.They were randomly assigned to the model group,total glucosides of paeony capsule group(TGP group,0.233 g/kg),Huoxue Jiedu Formula low-(15.925 g/kg),middle-(31.850 g/kg),and high-dose(62.335 g/kg)groups,with 15 mice in each group.The blank control and model groups received distilled water by gavage,and the other groups received respective drug doses by gavage once a day for 8 consecutive weeks.After 1,2,4,6,and 8 weeks of gavage,the salivary secretion volume of the mice was measured.The submaxillary gland index was calculated.Pathological changes in the submaxillary gland of mice were observed by hematoxylin-eosin staining.The expression of apoptosis-related factors Bcl-2 and survivin was detected by Western blotting and real-time fluorescence PCR.Results There was statistical significance in salivary secretion volume from aspects of between-group,time-point,and interaction effects(P<0.05).After 8 weeks of treatment,compared to the blank control group,salivary secretion volume,submaxillary gland index,and protein and mRNA expressions of Bcl-2 and survivin in the model group were decreased,while the pathological score grading increased(P<0.05).Compared to the model group,the TGP group and the Huoxue Jiedu Formula low-,medium-,and high-dose groups showed an increase in saliva secretion after 8 weeks of treatment and an increase in submaxillary gland index,Bcl-2,and survivin mRNA expression levels,and a decrease in pathological score levels.The protein expressions of Bcl-2 and survivin in the Huoxue Jiedu Formula medium-and high-dose groups were increased(P<0.05).Compared to the TGP group,salivary secretion volume and submaxillary gland index in the Huoxue Jiedu Formula low-dose group were lower at weeks 4 and 6,while the pathological score grading was higher(P<0.05).The pathological score grading in the Huoxue Jiedu Formula high-dose group was lower.The protein and mRNA expressions of Bcl-2 in the Huoxue Jiedu Formula low-and medium-dose groups were lower(P<0.05).Conclusion Huoxue Jiedu Formula may relieve the submaxillary gland lesions and increase the salivary secretion volume of the submaxillary gland in SS model mice by regulating the expressions of apoptosis-related factors such as Bcl-2 and survivin in the submaxillary gland.关键词
活血解毒方/干燥综合征/颌下腺/细胞凋亡/小鼠Key words
Huoxue Jiedu Formula/Sjögren syndrome/submaxillary gland/cell apoptosis/mice分类
医药卫生引用本文复制引用
王东峰,朱跃兰,侯秀娟,谷新怡,胡海,李方凯..活血解毒方对干燥综合征小鼠颌下腺细胞凋亡的影响研究[J].北京中医药大学学报,2024,47(12):1693-1701,9.基金项目
国家自然科学基金项目(No.81874438) （No.81874438）
北京中医药大学2019 年度自主课题青年教师项目(No.2019-JYB-JS-104) National Natural Science Foundation of China(No.81874438) （No.2019-JYB-JS-104）
