福建农业学报2024，Vol.39Issue(9)：1023-1034,12.DOI:10.19303/j.issn.1008-0384.2024.09.003
双孢蘑菇As2796菌株气生与贴生菌丝遗传差异的转录组分析
Differentiate Transcriptomes of Aerial and Creepy Agaricus bisporus Hyphae
摘要
Abstract
[Objective]Key genes associated with Agaricus bisporus of different mycelial morphology were analyzed.[Methods]As 2796,the main cultivated variety of A.bisporus,with aerial or creepy hyphae grown on same plate were collected for RNA transcriptome sequencing and comparison with reference.[Results]There were 965 differentially expressed genes(DEGs)between the aerial hyphae-A601 and the creepy hyphae-A602.According to the GO enrichment analysis,the hydrophobic DEGs were enriched in the fungal cell wall,such as AGABI2DRAFT_13655,AGABI2DRAFT_136569,and AGABI2DRAFT_193057 that belonged to the ABH3 protein gene family,with expressions significantly higher in A601 than in A602.Thus,they were postulated to be related to the production of aerial hyphae.In addition,the hydrophobic surface binding protein A(HsbA)-related gene,AGABI2DRAFT_194662,showed a FPKM expression of 0 in A601,but 10.24 in A602.Furthermore,8 enriched DEGs related to lipase isoenzymes exhibited a FPKM expression significantly higher in A601 than in A602.The SNP/InDel analysis indicated that the SNPs in A601 was 110 601 as opposed to 111 188 in A602,the most frequent mutation in SNPs was C:G>T:A,and that 2 374 common InDels were identified.[Conclusion]The transcriptomes and possibly associated genes and SNP sites in A.bisporus with different mycelial morphology obtained in this study would be of value for the mushroom germplasm evaluation and breeding.关键词
双孢蘑菇/As2796/贴生菌丝/气生菌丝/转录组测序/差异表达基因Key words
Agaricus bisporus/As 2796/creepy hypha/aerial hypha/transcriptome sequencing/differentially expressed genes分类
农业科技引用本文复制引用
戴建清,陈文智,曾志恒,蔡志欣,陈美元..双孢蘑菇As2796菌株气生与贴生菌丝遗传差异的转录组分析[J].福建农业学报,2024,39(9):1023-1034,12.基金项目
福建省科技计划公益类专项(2023R1033002) （2023R1033002）
国家现代农业产业技术体系建设专项(CARS-20) （CARS-20）
福建省种业创新与产业化工程项目(zycxny2021011) （zycxny2021011）
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021007) （XTCXGC2021007）
福建省农业科学院科技创新团队建设项目(CXTD2021007-1) （CXTD2021007-1）
