福建农业学报2024，Vol.39Issue(9)：1058-1068,11.DOI:10.19303/j.issn.1008-0384.2024.09.007
薄壳种油棕果实采后脂质成分转录代谢差异分析
Differentiation in Post-harvest Lipid Metabolism of Oil Palm Fruits
摘要
Abstract
[Objective]Mechanism and accumulation of lipid synthesis in thin-shelled oil palm fruits was investigated for breeding of variety resistant to fat rancidity.[Method]Fruits of thin-shelled oil palm freshly harvested 185d after pollination(T1),24h post-harvest(T2),and 36h post-harvest(T3)were collected for LC-MS/MS and RNA-seq determination and analysis on lipid metabolites and differentially expressed genes in mesocarp of oil palm fruits as the lipid oxidation taking place.[Result]In the fruit development,5 lipid classes,23 lipid subclasses,and 520 monomer molecules in mesocarp of the oil palm fruits were identified.It is well known that the hydrolysis of phosphatidylcholine(PC)is a lipid oxidation and the hydrolyzed glycerophosphate choline(GPC)affects PC,lipophosphatase(LPP)promotes synthesis of phosphates and glycerophospholipids,and the expression of chlorophyll relates to chlorophyll content.This study found that the aldehyde dehydrogenase(ALDH7A1 and ALDH2),monoacylglycerol lipase(MGL),phospholipase A1(PLA1),and glycerophosphodiester phosphodiesterase(GDPD1)significantly negatively correlated with glycerophospholipids,such as diacylglycerol trimethyl homoserine(DGTS),phosphatidic acid(PA),phosphatidylinositol(PI),phosphatidylcholine(PC),phosphatidylglycerol(PG),phosphatidylethanolamine(PE),but significantly positively correlated with palmitic acid,while GDPD1,LPP,and digalactosylglycerol synthase(DGD1)significantly positively correlated with the glycerophospholipids,such as DGTS,PA,PI,PC,PG,PE,but negatively correlated with palmitic acid,whereas MGL monoglyceride(MG)extremely significantly positively correlated with linoleic acid(LA)but significantly negatively correlated with ceramide(Cer),and DGD1 and LPP significantly negatively correlated with MG and LA,but significantly positively correlated with Cer.[Conclusion]It appeared that ALDH7A1,ALDH2,PLA1,and MGL inhibited the glycerophospholipids synthesis but promoted the synthesis of fatty acids such as palmitic acid,while DGD1,LPP,and GDP1 enhanced the synthesis of glycerophospholipids but retarded that of palmitic and other fatty acids.关键词
油棕/酸败/脂质/代谢组学/转录组学Key words
Oil palm fruit/rancidity/lipid/metabolomics/transcriptomics分类
农业科技引用本文复制引用
李欣瑜,刘小玉,李启黉,周丽霞,李睿,付登强,曹红星..薄壳种油棕果实采后脂质成分转录代谢差异分析[J].福建农业学报,2024,39(9):1058-1068,11.基金项目
海南省国际科技合作研发项目(GHYF2024019) （GHYF2024019）
中国热带农业科学院基本科研业务费专项(1630152023007) （1630152023007）
