福建农业学报2024，Vol.39Issue(9)：1094-1101,8.DOI:10.19303/j.issn.1008-0384.2024.09.011
福建省大豆胶孢炭疽病菌对吡唑醚菌酯的抗性风险分析
Risk Assessment on Resistance to Pyraclostrobin of Colletotrichum gloeosporioides
摘要
Abstract
[Objective]Drug sensitivity to and cross-resistance of pyraclostrobin with other fungicides of Colletotrichum gloeosporioides that caused anthracnose in soybeans in Fujian Province were investigated.[Methods]Sensitivity to pyraclostrobin of 112 strains of C.gloeosporioides isolated from 5 regions in the province in 2023 was determined using the mycelial production rate method.Resistant mutants of the pathogen were obtained by fungicide domestication to determine their genetic stability,fitness,and possible cross-resistance to 4 fungicides.[Results]The EC50 of pyraclostrobin for the 112 C.gloeosporioides strains ranged 0.0682µg·mL-1 to 1.0309µg·mL-1 with a coefficient of variation of 15.12 and averaged(0.203 6±0.121 5)µg·mL-1 as the sensitivity baseline.The distribution of the sensitivity frequency was in a continuous unimodal function consistent with the continuous skewed normal distribution.Induced from two wild C.gloeosporioides,4 moderate resistant strains and one low resistant strain were secured at a frequency of 8.3×10-4.The mutants stably inherited the drug resistance traits but differed from their parents in temperature sensitivity and were slightly lower on mycelial growth rate,sporulation ability,and pathogenicity.In the field,they might sacrifice other natural competitive advantages as a fitness cost in exchange for the drug resistance.Pyraclostrobin showed no cross-resistance with carbendazim,difenoconazole,and prochloraz but did with the same type of fungicide,picoxystrobin.[Conclusion]Although the C.gloeosporioides isolated in Fujian were relatively high in sensitivity to pyraclostrobin,they represented merely a moderate drug resistance risk.Nonetheless,in practice,it was plausible to blend pyraclostrobin or apply alternatively with fungicides such as carbendazim,difenoconazole,and prochloraz to delay such potential drawback.关键词
大豆胶孢炭疽菌/吡唑醚菌酯/敏感基线/抗性风险/交互抗性Key words
Colletotrichum gloeosporioides/pyraclostrobin/sensitivity baseline/resistance risk/cross-resistance分类
农业科技引用本文复制引用
阮宏椿,黄艳琴,陈巧红,陈文乐,兰成忠..福建省大豆胶孢炭疽病菌对吡唑醚菌酯的抗性风险分析[J].福建农业学报,2024,39(9):1094-1101,8.基金项目
福建省科技计划公益类专项(2021R1024002) （2021R1024002）
福建省农业科学院对外合作项目(DWHZ-2023-04) （DWHZ-2023-04）
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021011、XTCXGC2021017) （XTCXGC2021011、XTCXGC2021017）
