番茄抗多种病害种质资源的筛选与鉴定OACSTPCD

This experiment used multi gene aggregation technology to hybridize the maternal material tomato 13485(including Tm-1、Mi-1)with the paternal material tomato high-level inbred line 13065(including Ty-2、Ty-3、I-2、Ph-2、Ph-3).Using molecular marker assisted selection(MAS)technology,a total of 653 individu-al plants were recorded in the F6 population of hybrid offspring,and disease resistance genes and artificial in-oculation were detected during the seedling stage,finally,12 materials containing 7 homozygous disease resist-ance genes were obtained to resist 5 diseases,10 materials resistant to 5 diseases and containing 7 disease re-sistant genes that are homozygous except Ty-3,5 materials resistant to 5 diseases and containing 7 disease re-sistant genes that are homozygous except for Ph-3,a homozygous material with Ph-3 resistance to five diseases and no Ph-2 resistance gene,4 homozygous materials with Ty-3 genes but Ty-2 genes resistant to five disea-ses,4 homozygous materials with Ty-3 resistance to 5 diseases but no Ty-2 resistance genes,the molecular de-tection results of the above materials have a consistency of over 85%with the field inoculation identification.Therefore,this study has certain practical significance for the breeding of tomato multi resistant varieties.