辽宁农业科学Issue(6)：1-7,7.DOI:10.3969/j.issn.1002-1728.2024.06.001
番茄抗多种病害种质资源的筛选与鉴定
Screening and Identification of Tomato Germplasm Resources Resistant to Various Diseases
摘要
Abstract
This experiment used multi gene aggregation technology to hybridize the maternal material tomato 13485(including Tm-1、Mi-1)with the paternal material tomato high-level inbred line 13065(including Ty-2、Ty-3、I-2、Ph-2、Ph-3).Using molecular marker assisted selection(MAS)technology,a total of 653 individu-al plants were recorded in the F6 population of hybrid offspring,and disease resistance genes and artificial in-oculation were detected during the seedling stage,finally,12 materials containing 7 homozygous disease resist-ance genes were obtained to resist 5 diseases,10 materials resistant to 5 diseases and containing 7 disease re-sistant genes that are homozygous except Ty-3,5 materials resistant to 5 diseases and containing 7 disease re-sistant genes that are homozygous except for Ph-3,a homozygous material with Ph-3 resistance to five diseases and no Ph-2 resistance gene,4 homozygous materials with Ty-3 genes but Ty-2 genes resistant to five disea-ses,4 homozygous materials with Ty-3 resistance to 5 diseases but no Ty-2 resistance genes,the molecular de-tection results of the above materials have a consistency of over 85%with the field inoculation identification.Therefore,this study has certain practical significance for the breeding of tomato multi resistant varieties.关键词
番茄/多基因聚合/多抗/分子标记Key words
Tomato/Polymerization/Multi-resistant/Molecular marker分类
农业科技引用本文复制引用
李冬艳,宋承泽,许向阳..番茄抗多种病害种质资源的筛选与鉴定[J].辽宁农业科学,2024,(6):1-7,7.基金项目
黑龙江省"百千万"工程科技重大专项(2019ZX16B02) （2019ZX16B02）
