农业环境科学学报2024，Vol.43Issue(11)：2657-2667,11.DOI:10.11654/jaes.2024-0313
养猪污水多级净化系统底泥细菌群落结构分析
Analysis of microbial community structure in the sediment of multi-stage purification ponds for swine wastewater treatment systems
摘要
Abstract
To explore the structure of microbial communities in the sediment of multi-stage purification pools within pig farm wastewater treatment systems and their interactions with environmental factors in the sediment,a multi-stage biological treatment and purification system was constructed,including Myriophyllum verticillatum L(T1),Pistia stratiotes L(T2),Azolla pinnata(T3),Misgurnus anguillicaudatus(T4),Pampus argenteus(T5),and Nelumbo nucifera Gaertn(T6).After six months of operation,the results of determining the physicochemical indicators of the sediment and the structure of the microbial community showed that throughout all purification stages,the Proteobacteria phylum consistently dominated the sediment;the Actinobacteriota and Firmicutes were more abundant in the initial treatment stage(T1)and significantly reduced in subsequent treatment pools.The relative abundance of Cyanobacteria,Acidobacteriota,and Desulfobacterota fluctuated greatly.In-depth analysis revealed that organic matter and total phosphorus were key environmental factors affecting the structure of microbial communities,with the content of organic matter showing a significant positive correlation with the abundance of unclassified genera of Syntrophales,Gaiellales,and Spirochaeta.Additionally,the denitrifying bacterium Candidatus Competibacter played a central role in the nitrogen cycle of the system.Therefore,the purification efficiency of the sewage treatment system can be improved by screening and using these specific functional bacteria for bioenhancement.关键词
养猪污水/净化池/高通量测序/微生物群落/环境因子Key words
swine wastewater/purification pond/high-throughput sequencing/microbial community/environmental factor分类
资源环境引用本文复制引用
陈钟佃,罗立津,周柳婷,黄小云,聂毅磊,黄秀声,翁伯琦,冯德庆..养猪污水多级净化系统底泥细菌群落结构分析[J].农业环境科学学报,2024,43(11):2657-2667,11.基金项目
福建省科研院所公益类专项(2022R1021005) （2022R1021005）
福建省人民政府-中国农业科学院"5511"协同创新工程项目(XTCXGC2021010) （XTCXGC2021010）
福建省农业科学院项目(CXTD2021009-1,YDXM202205)Special Fund for Scientific Research on Public Causes of Fujian Province(2022R1021005) （CXTD2021009-1,YDXM202205）
"5511"Collaborative Innovation Project of People′s Government of Fujian Province and Chinese Academy of Agricultural Sciences(XTCXGC2021010) （XTCXGC2021010）
Fujian Academy of Agricultural Sciences Project(CXTD2021009-1,YDXM202205) （CXTD2021009-1,YDXM202205）
