中国兽医科学2024，Vol.54Issue(12)：1563-1568,6.DOI:10.16656/j.issn.1673-4696.2024.0216
宿主蛋白VDAC1抑制口蹄疫病毒复制的机制研究
Study on mechanism of host protein VDAC1 inhibiting replication of foot-and-mouth disease virus
摘要
Abstract
foot-and-mouth disease is a highly contagious and severe illness triggered by the foot-and-mouth disease virus(FMDV)in even-toed ungulates like pigs,cattle,and sheep.The nonstructural protein 3Dpol of FMDV is crucial for the virus's replication and its role in causing the disease.In order to investigate the mechanism of host protein interaction with 3Dpol protein,this study firstly identi-fied the interaction between VDAC1 and FMDV 3Dpol using yeast two-hybrid and immunoprecipitation exper-iments,and then found the overexpression of VDAC1 and inhibitor experiments showed that VDAC1 inhibit-ed the expression of 3Dpol protein through autophagy pathway.Finally,it was found that down-regulation of VDAC1 had a promoting effect on FMDV replication,suggesting that VDAC1 was important in the replica-tion and pathogenicity of FMDV.This study reveals a new mechanism of host protein inhibition of FMDV replication,and the results provide a new direction and target for the prevention and control of FMD.关键词
口蹄疫病毒/3Dpol/宿主蛋白VDAC1/自噬Key words
foot-and-mouth disease virus/3Dpo1/VDAC1/autophagh分类
农业科技引用本文复制引用
马柯,薛巧,刘鹏飞,朱紫祥,李宏斌,刘会胜,郑海学..宿主蛋白VDAC1抑制口蹄疫病毒复制的机制研究[J].中国兽医科学,2024,54(12):1563-1568,6.基金项目
国家重点研发计划项目(2021YFD1801300) （2021YFD1801300）
国家生猪产业体系项目(CARS-35) （CARS-35）
国家资助博士后研究人员计划(GZC20233064) （GZC20233064）
中央高校基本科研业务费专项资金项目 （）
国家生猪技术创新中心先导科技项目(NCTIP-XD/C03) （NCTIP-XD/C03）
甘肃省科技重大专项(22ZD6NA001) （22ZD6NA001）
"十四五"广东省农业科技创新十大主攻方向"揭榜挂帅"项目(2022SDZG02) （2022SDZG02）
中国农业科学院科技创新工程项目(CAAS-ASTIP-2022-LVRI) （CAAS-ASTIP-2022-LVRI）
中国农业科学院基础科学中心重大任务(CAAS-CSLPDC-202302) （CAAS-CSLPDC-202302）
