中国兽医科学2024，Vol.54Issue(12)：1595-1600,6.DOI:10.16656/j.issn.1673-4696.2024.0210
牛病毒性腹泻病毒NM-1株的分离鉴定及基因序列分析
Isolation,identification and gene sequence analysis of bovine viral diarrhea virus NM-1 strain
摘要
Abstract
To investigate the prevalence of bovine viral diarrhea and the genetic characteristics of BVDV epidemic strains in Inner Mongolia area,In this study,fecal samples of diarrheal calves from a dairy farm in Inner Mongolia area were collected,filtered and sterilized,and inoculated into bovine kidney cells(MDBK).After 5 generations of blind transmission,the cytopathic changes were observed and identified by RT-PCR.The results showed that a cytopathic bovine viral diarrhea strain was successfully isolated and named BVDV-NM-1 strain.The typical BVDV virion morphology of BVDV-NM-1 was observed by electron microscopy.The 5'-UTR gene sequence of BVDV-NM-1 strain was amplified and compared with that of BVDV-1 type.The homology of NM-1 strain with BVDV-1v subtype was 96％.It laid the foundation for better prevention and control of bovine viral diarrhea in Inner Mongolia area.关键词
牛病毒性腹泻病毒/分离鉴定/序列分析Key words
bovine viral diarrhea virus(BVDV)/isolation and identification/sequence analysis分类
农业科技引用本文复制引用
刘方圆,赵传芳,张东亮,吕文雪,吕爽,王春霞,许皓,袁帅,陈雪,徐凤宇..牛病毒性腹泻病毒NM-1株的分离鉴定及基因序列分析[J].中国兽医科学,2024,54(12):1595-1600,6.基金项目
吉林省科技厅创新平台(基地)和人才专项(20230508009RC) （基地）
