中国兽医科学2024，Vol.54Issue(12)：1617-1626,10.DOI:10.16656/j.issn.1673-4696.2024.0199
江苏省部分地区鸡滑液囊支原体的分离鉴定及致病性和耐药性评价
Isolation and identification of Mycoplasma synoviae from chickens in some areas of Jiangsu Province and evaluation of pathogenicity and aitibiotics resistance
摘要
Abstract
In order to explore the genotyping,pathogenicity,and aitibiotics susceptibility of My-coplasma synoviae(MS)in selected areas of Jiangsu Province,this study isolated and characterized MS strains.The isolates were identified through observation of colony morphology,serological testing,16S rRNA sequencing,and vlhA-PRR typing.Additionally,SPF chicken challenge tests and antimicrobial susceptibility tests were performed.The results showed that three suspected MS strains were isolated from the collected samples,exhibiting characteristic'fried egg'colonies on solid medium.The 16S rRNA sequence analysis showed that each isolate had 99.9％similarity with MS.Furthermore,plate agglutina-tion test showed that it had agglutination reaction with MS-positive serum and MS-infected serum,but did not react with negative serum of SPF chicken,so it was confirmed that the isolates were MS.The SPF chicken challenge test showed that the three isolates caused obvious joint,foot pad swelling and mild air sac inflammation in chickens,and the symptoms of the joint challenge group were the most obvious.The MIC test showed that,compared to the standard strain MS WVU1853,the three isolates displayed a sig-nificantly reduced sensitivity to tilmicosin and enrofloxacin,as well as varying degrees of reduced sensitivity to tylosin,kitasamycin,tiamulin,lincomycin,florfenicol,and spectinomycin.However,they retained a high sensitivity to valnemulin hydrochloride,doxycycline,oxytetracycline,and tyl-valosin.This study provides scientific basis for local prevention and control of MS infections,offers methodological guidance for the isolation,identification,and aitibiotics selection of clinical MS cases,and lays a research foundation for the establishment of a challenge model in MS vaccine evaluation.关键词
滑液囊支原体/分离鉴定/vlhA基因分型/致病性/药物敏感性Key words
Mycoplasma synoviae/isolation and identification/genotyping of vlhA gene/pathogenici-ty/aitibiotics susceptibility分类
农业科技引用本文复制引用
赵鹿如,祁晶晶,张开琴,王芷洋,叶丽君,田明星,鲍衍清,唐小川,王少辉..江苏省部分地区鸡滑液囊支原体的分离鉴定及致病性和耐药性评价[J].中国兽医科学,2024,54(12):1617-1626,10.基金项目
国家重点研发计划项目(2023YFD1800602) （2023YFD1800602）
国家自然科学基金项目(31902244) （31902244）
中国农业科学院科技创新工程项目(CAAS-ASTIP-2021-SHVRI) （CAAS-ASTIP-2021-SHVRI）
