北华大学学报（自然科学版）2025，Vol.26Issue(1)：36-41,6.DOI:10.11713/j.issn.1009-4822.2025.01.006
葫芦素B改善脂多糖诱导C2C12肌管萎缩作用机制
Effect and Mechanism of Cucurbitacin B on Improving Lipopolysaccharide-induced C2C12 Myotube Atrophy
摘要
Abstract
Objective To explore the influence of Cucurbitacin B(CuB)on LPS-induced C2C12 myotube atro-phy and skeletal muscle atrophy in C57BL/6 mice.Methods Protein expression levels were determined by Western blot,cell morphological alterations were observed using H&E staining,and the degree of skeletal muscle atrophy was evaluated by measuring the grip strength of mice and the mass of gastrocnemius and tibialis anterior muscles.Results Western blot experiment indicated that LPS was capable of inducing the atrophy of C2C12 myotubes(P<0.01),while treatment with CuB(0.3～3.0 μmol/L)could promote the expression of myotube differentiation-related proteins(MyHC)and muscle synthesis-related proteins,and reduce the release of proinfla-mmatory factors(IL-1β,COX-2)(P<0.001).The results of H&E staining assay confirmed morphologically that CuB could effectively ameliorate the myotube atrophy stimulated by LPS.Additionally,the animal experiments revealed that LPS could lead to a significant decline in the grip strength of C57BL/6 mice and the mass of gastrocnemius and tibialis anterior muscles,and the symptoms were improved after treatment with CuB(P<0.05).Conclusion CuB holds potential application value in treatment of skeletal muscle atrophy.It offers a theoretical foundation for the development of lead compounds from natural medicinal sources and pioneers a novel thinking for the treatment of inflammatory skeletal muscle atrophy.关键词
葫芦素B/骨骼肌萎缩/抗炎Key words
Cucurbitacin B/skeletal muscle atrophy/anti-inflammatory分类
医药卫生引用本文复制引用
曹晟,左红香,丁嘉,肖樾笛,杨博云,邰屹,刘欣哲,邢月,李明月,马娟..葫芦素B改善脂多糖诱导C2C12肌管萎缩作用机制[J].北华大学学报（自然科学版）,2025,26(1):36-41,6.基金项目
吉林省教育厅科学技术研究项目(JJKH20220561KJ) （JJKH20220561KJ）
吉林省科技发展计划项目(YDZJ202201ZYTS169). （YDZJ202201ZYTS169）
