北京林业大学学报2024，Vol.46Issue(12)：53-62,10.DOI:10.12171/j.1000-1522.20230341
野生与栽培生境下沙氏鹿茸草根际土壤真菌群落多样性比较
Comparison of fungal community diversity in rhizosphere soil of Monochasma savatieri in wild and cultivated habitats
摘要
Abstract
[Objective]In order to solve the problem of poor growth of Monoctasma savatieri in artificial cultivation compared with wild habitats,the reason for poor growth in artificial cultivation was explored from the perspective of rhizosphere soil fungi.[Method]Study subjects were fungi in rhizosphere soil of Monochasma savatieri in one artificially cultivated habitat and three wild habitats,namely Camellia oleifera forest,Gardenia jasminoides forest,and Pinus massoniana plantation.A field survey,ITS high-throughput sequencing,RDA,Mantel analysis were conducted to compare the population characteristics of fungi in rhizosphere soil of Monochasma savatieri.[Result]A total of 15 phyla,50 classes,139 orders,299 families,and 644 genera of fungi were detected in the rhizosphere soil of Monochasma savatieri in the four habitats,with the dominant phyla being Ascomycota and Basidiomycota.There were a total of 27 dominant genera.The dominant fungal genus in rhizosphere soil of Monochasma savatieri under Pinus massoniana plantation and Camellia oleifera forest was Archaeorhizomyces,while the dominant fungal genus in rhizosphere soil of Gardenia jasminoides forest was Saitozyma.Under artificial cultivation conditions,the dominant fungal genus was Leucoagaricus.There were significant differences between fungal community structures of rhizosphere soil of Monochasma savatieri under artificial cultivation and wild habitats(Gardenia jasminoides forest,Camellia oleifera forest,and Pinus massoniana plantation).And its richness index(Chao1 index)was significantly lower than that of Gardenia jasminoides habitat.The fungal community structure was mainly affected by alkali-hydrolysable nitrogen and organic matter in soil,and alkali-hydrolysable nitrogen was also a major environmental factor affecting genus Leucoagaricus.[Conclusion]There is a significant difference in composition of soil fungal communities between cultivated and wild habitats of Monochasma savatier.The fungal community structure is mainly affected by alkali-hydrolysable nitrogen and organic matter in soil.So the growth of Monochasma savatieri can be regulated by measures such as applying nitrogen fertilizer.关键词
栽培/群落多样性/土壤真菌/生境/根际土壤/沙氏鹿茸草/N肥Key words
cultivation/community diversity/soil fungi/habitat/rhizosphere soil/Monochasma savatieri/nitrogen fertilizers分类
农业科技引用本文复制引用
罗桂生,刘素贞,李峰卿,曾满生,邹玉玲,朱小毛,周新华,朱柯帆..野生与栽培生境下沙氏鹿茸草根际土壤真菌群落多样性比较[J].北京林业大学学报,2024,46(12):53-62,10.基金项目
中国林科院基本科研业务费专项资金(CAFYBB2022MB005),国家重点研发计划(2023YFD2200903),新余市科技计划项目余科发(2023)32号. （CAFYBB2022MB005）
