北京林业大学学报2024，Vol.46Issue(12)：82-91,10.DOI:10.12171/j.1000-1522.20240288
干燥和储存方式对植物叶片次生代谢物含量的影响
Effects of drying and storage methods on contents of secondary metabolites in plant leaves
摘要
Abstract
[Objective]This study aimed to provide guidance on pretreatment methods for plant leaves during field sampling,preservation,and experimentation by detecting changes in the contents of total phenol,flavonoid,and tannin in plant leaves after different pretreatments.[Method]The leaves of herbaceous plants,shrubs,broadleaved trees,and coniferous trees were selected as research objects.The drying pretreatment methods included vacuum freeze-drying,oven drying,microwave drying,natural sun drying,and natural shade drying.The storage pretreatment methods involved storage temperatures of-20 and-80℃and storage durations of 15 and 30 d.The effects of different pretreatment methods on contents of total phenol,flavonoid,and tannin in plant leaves were compared.[Result](1)After different drying treatments in the leaves of 8 plant species,the maximum content of total phenol in leaves mostly appeared in vacuum freeze-drying and microwave drying;the maximum flavonoid content of leaves mostly appeared in vacuum freeze-drying,oven drying and microwave drying;the maximum tannin content of leaves mostly appeared in vacuum freeze-drying and microwave drying.(2)The contents of total phenol,flavonoid and tannin in leaves of 8 species decreased with the increase of storage time,and the contents of secondary metabolites measured at-80℃were mostly higher than those stored at-20℃.[Conclusion]In experiments detecting the content of foliar secondary metabolites,we recommend to complete the measurement as soon as possible after sample collection.Vacuum freeze-drying or microwave drying methods can be used for sample drying pretreatment.If long-distance transportation is required after sampling from field experiment sites,and storage conditions at-80℃cannot be guaranteed,short-term transportation and storage at-20℃is acceptable for measurement of the content of foliar secondary metabolites.Microwave drying,as a fast,safe,and efficient drying method,has strong applicability in the pretreatment of plant samples in laboratories.关键词
次生代谢物/预处理方法/植物叶片/干燥方式/储存方式Key words
secondary metabolites/pretreatment methods/plant leaves/drying methods/storage methods分类
农业科技引用本文复制引用
梁昌明,田地,赵常提,陈冰瑞,莫仪恩,夏青霖,霍怡妃..干燥和储存方式对植物叶片次生代谢物含量的影响[J].北京林业大学学报,2024,46(12):82-91,10.基金项目
北京市自然科学基金项目(5222018). （5222018）
