成都大学学报（自然科学版）2024，Vol.43Issue(4)：349-354,6.DOI:10.3969/j.issn.1004-5422.2024.04.003
激素对银杏叶愈伤组织状态的影响及悬浮培养体系的建立
Effect of Hormones on Callus Status of Ginkgo Biloba Leaves and Establishment of Suspension Culture System
摘要
Abstract
By using Ginkgo Biloba leaves as materials,the effects of different combinations and proportions of auxin(NAA,2,4-D),cytokinin(6-Ba,KT)and different culture media on callus state were studied so as to screen out the best combination of hormones and culture media suitable for callus induction of Gink-go Biloba leaves.Excellent suspension cell culture system of Ginkgo Biloba was established after the sub-culture of fine callus.The results showed that the callus induction rate of NAA and 6-BA combination,NAA and KT combination was higher than that of 2,4-D and 6-BA combination,2,4-D and KT combina-tion.And the induced callus state was better.The browning degree of callus induced by different hormone combinations was different.The callus induced by NAA and 6-BA combination,NAA and KT combination had no browning,and the browning degree of 2,4-D and 6-BAcombination,2,4-D and KT combination was more serious.When NAA and KT was combined,NAA concentration was 1.5 mg/L and KT concen-tration was 1.0～1.5 mg/L,the induction rate of callus was high and the callus state was excellent.When NAA and 6-BA was combined,and NAA concentration was 1.5 mg/L and 6-BA concentration was 0.5～1.0 mg/L,the callus induction rate was high.The suitable culture medium for inducing Ginkgo Biloba leaves callus were N6 and MS.In conclusion,the suitable combinations of hormones for inducing Ginkgo Biloba callus are NAA and KT,NAA and 6-BA.Excellent callus(loose,granular,yellow-green,no brown-ing,no water stain)can be induced by the combinations on N6 or MS culture medium.After subculture,the suspension cell culture system of Ginkgo Biloba can be established.关键词
银杏叶/愈伤组织/状态/悬浮培养/褐变Key words
Ginkgo Biloba/callus/status/suspension culture/browning分类
医药卫生引用本文复制引用
李玲玲..激素对银杏叶愈伤组织状态的影响及悬浮培养体系的建立[J].成都大学学报（自然科学版）,2024,43(4):349-354,6.基金项目
重庆市涪陵区科技计划项目(FLKJ2016ABB1053) （FLKJ2016ABB1053）
重庆市教委高等教育教学改革研究项目(Z213126) （Z213126）
