电化学（中英文）2024，Vol.30Issue(10)：18-27,10.DOI:10.61558/2993-074X.3483
基于铂纳米颗粒碰撞电化学用于快速检测乳腺癌MCF-7细胞
Platinum Nanoparticle-based Collision Electrochemistry for Rapid Detection of Breast Cancer MCF-7 Cells
摘要
Abstract
Cancer metastasis is the leading cause of death in cancer patients worldwide and one of the major challenges in treating cancer.Circulating tumor cells(CTCs)play a pivotal role in cancer metastasis.However,the content of CTCs in peripheral blood is minimal,so the detection of CTCs in real samples is extremely challenging.Therefore,efficient enrichment and early detection of CTCs are essential to achieve timely diagnosis of diseases.In this work,we con-structed an innovative and sensitive single-nanoparticle collision electrochemistry(SNCE)biosensor for the detection of MCF-7 cells(human breast cancer cells)by immunomagnetic separation technique and liposome signal amplifi-cation strategy.Liposomes embedded with platinum nanoparticles(Pt NPs)were used as signal probes,and home-made gold ultramicroelectrodes(Au UME)were used as the working electrodes.The effective collision between Pt NPs and UME would produce distinguishable step-type current.MCF-7 cells were accurately quantified according to the relationship between cell concentration and collision frequency(the number of step-type currents generated per unit time),realizing highly sensitive and specific detection of MCF-7 cells.The SNCE biosensor has a linear range of 10 cells.mL-1 to 105 cells mL-1 with a detection limit as low as 5 cells.mL-1.In addition,the successful detection of MCF-7 cells in complex samples showed that the SNCE biosensors have great potential for patient sample detection.关键词
循环肿瘤细胞/单颗粒碰撞电化学/免疫磁分离/脂质体/铂纳米颗粒Key words
Circulating tumor cells/Single-nanoparticle collision electrochemistry/Immunomagnetic separation/Liposome/Platinum nanoparticles引用本文复制引用
秦富星,李明珂,周汇龙,文为,张修华,王升富,伍珍..基于铂纳米颗粒碰撞电化学用于快速检测乳腺癌MCF-7细胞[J].电化学（中英文）,2024,30(10):18-27,10.基金项目
This work was supported by the National Natu-ral Science Foundation of China(Nos.22274037,22376055 and 21904032),and the Natural Science Foundation of Hubei Province(2022CFB383). （Nos.22274037,22376055 and 21904032）
