海南医学院学报2024，Vol.30Issue(24)：1841-1847,7.DOI:10.13210/j.cnki.jhmu.20240719.001
胰管捆扎构建小鼠胰腺炎模型的实验研究
Experimental study on mouse model of pancreatitis constructed by ligation of pancreatic duct
摘要
Abstract
Objective:To compare the pancreatic pathological characteristics of acute pancreatitis(AP)and chronic pancreati-tis(CP)models constructed by pancreatic duct ligation with those constructed solely by cerulein.Methods:A single intraperitone-al injection of cerulein(50 µg/kg/body weight)was administered 48 h after duct ligation.The mice sacrificed 3 d after ligation were designated as the AP group,while those sacrificed 21 d after ligation were designated as the CP group.The AP model in-duced by cerulein was established by continuous intraperitoneal injection of cerulein 7 times(50 μg·kg-1·h-1),mice were sacrificed 24 h after first injection.The CP model induced by cerulein was established by continuous intraperitoneal injection of cerulein on the 1st,3rd,and 6th day of the week,6 times a day(50 μg·kg-1·h-1),for a total of 6 weeks,and mice were sacrificed 5 d after last injection.H&E staining is used to detect the pathological morphology of the pancreas,immunohistochemistry staining is used to detect the expression of inflammatory mediators and fibrosis markers,and Masson staining is used to detect pancreatic fibrosis.Results:Compared to the AP model induced by cerulein,those constructed by ligation of pancreatic duct exhibited a higher patho-logical score and higher expression of inflammatory mediators IL-6 and TNF-α.Compared to the CP model induced by cerulein,those constructed by ligation of pancreatic duct exhibited more obvious loss of pancreatic acinar cells,larger proportion of fibrosis,and higher expression of fibrotic markers including α-SMA and CTGF.Conclusion:Compared to the pancreatitis model induced by cerulein,pancreatitis model constructed by ligation of pancreatic duct has severer pancreatic damage,while the CP model con-structed by it has more obvious pancreatic acinar loss and fibrosis,with lower costs in time and economy than those induced by ceru-lein.关键词
急性胰腺炎/慢性胰腺炎/雨蛙素/捆扎Key words
Acute pancreatitis/Chronic pancreatitis/Cerulein/Ligation分类
医药卫生引用本文复制引用
彭程,涂广平,李志强..胰管捆扎构建小鼠胰腺炎模型的实验研究[J].海南医学院学报,2024,30(24):1841-1847,7.基金项目
This study was supported by the Yluth Project of the National Natural Science Foundation of China(82100688) 国家自然科学基金青年项目(82100688) （82100688）
