海南医学院学报2024，Vol.30Issue(24)：1848-1858,11.DOI:10.13210/j.cnki.jhmu.20240718.001
MEBT/MEBO通过调控TXNIP/NLRP3/Caspase-1信号通路促进糖尿病创面愈合
MEBT/MEBO promotes diabetic wound healing by regulating TXNIP/NLRP3/Caspase-1 pathway
摘要
Abstract
Objective:To investigate the effect of moist exposed burn therapy/moist exposed burn ointment(MEBT/MEBO)on the expression of TXNIP/NLRP3/Caspase-1 pathway in diabetic wounds.Methods:A total of 48 male Wistar rats were ran-domly divided into the control group,the model group,the MEBO group and the rb-bFGF group.The acute wound model was es-tablished in the control group,and the diabetic wound model was established in the other groups.Then the MEBO group and the rb-bFGF group were treated with moist burn ointment and recombinant bovine basic fibroblast growth factor,respectively.The control group and the model group were treated with normal saline.The wound healing and the expression of TXNIP,NLRP3,and Caspase-1 in each group were observed by H&E staining,immunofluorescence method,and real-time qPCR on the 3rd,7th,and 14th day.Results:⑴ On the 3rd day of treatment,the wound healing rate of the control group was higher than that of the model group,and the difference was statistically significant(P<0.05).On the 7th and 14th day of treatment,the wound healing rates of the control group,MEBO group,and rb-bFGF group were higher than those of the model group(P<0.05).⑵ On the 3rd,7th,and 14th day of treatment,the expression levels of TXNIP and NLRP3 in the control group,MEBO group,and rb-bF-GF group were lower than those in the model group(P<0.05).On the 3rd,7th and 14th day of treatment,the expression levels of Caspase-1 in the control group and MEBO group were lower than those in the model group(P<0.05).Conclusion:MEBT/MEBO can inhibit the expression of TXNIP/NLRP3/Caspase-1 pathway,reduce the inflammatory response of diabetic wound,and thus promote the healing of diabetic wound.关键词
湿润暴露疗法/湿润烧伤膏(MEBT/MEBO)/糖尿病创面/TXNIP/NLRP3/Caspase-1Key words
Moist exposed burn therapy/moist exposed burn ointment/Diabetic wound/TXNIP/NLRP3/Caspase-1分类
医药卫生引用本文复制引用
姚明哲,竺仕林,李文武,杨雅量,葛星月,唐乾利..MEBT/MEBO通过调控TXNIP/NLRP3/Caspase-1信号通路促进糖尿病创面愈合[J].海南医学院学报,2024,30(24):1848-1858,11.基金项目
This study was supported by the National Natural Science Foundation Project(81774327) （81774327）
Guangxi Special Expert Project[Guangxi Talent Pass Word(2019)No.13] （2019）
The"139"Plan for Senior and Secondary Backbone Talents in Guangxi Medicine[Gui Wei Ke Jiao Fa(2018)No.22] （2018）
Youjiang Medical University for Nationalities Graduate Innovation Project(YXCXJH2023003) 国家自然科学基金面上项目(81774327) （YXCXJH2023003）
广西特聘专家项目[桂人才通字(2019)13号] （2019）
广西医学高层次骨干人才"139"计划培养人选培养专项资助[桂卫科教发(2018)22号] （2018）
右江民族医学院研究生创新计划项目(YXCXJH2023003) （YXCXJH2023003）
