欣胃颗粒调控METTL6/CyclinD1通路对胃癌细胞增殖、凋亡和细胞周期的影响OA北大核心CSTPCD

Objective:To investigate the effects of Xinwei granules on the METTL16/CyclinD1 pathway and proliferation,apoptosis,and cell cycle of gastric cancer cells,and to elucidate the molecular mechanisms underlying its therapeutic potential in gastric cancer.Methods:Cell viability of the MKN-45 gastric cancer cell line was assessed using the CCK-8 assay following treat-ment with various concentrations of gastric-derived granules.Flow cytometry was employed to analyze apoptosis and cell cycle dis-tribution.Western blot and RT-qPCR analyses were used to measure the expression of key molecules in the METTL16/CyclinD1 pathway.Spot hybridization was performed to detect m6A methylation modifications of CyclinD1.Results:Compared to the con-trol group,gastric-derived granules significantly increased apoptosis in MKN-45 cells,elevated the proportion of cells in the G1 phase,reduced the proportion in the S phase,and downregulated the expression of CyclinD1,CDK4,E2F1,and METTL16 proteins as well as the p-Rb/Rb ratio(P<0.01).Additionally,gastric-derived granules significantly decreased the mRNA expres-sion levels of METTL16 and CyclinD1,and inhibited CyclinD1 m6A methylation modifications(P<0.01).Conclusion:Xinwei granules exert their effects by inhibiting METTL16-mediated m6A methylation of CyclinD1,thereby blocking the transition from the G1 phase to the S phase,suppressing cell proliferation,and promoting apoptosis.