海南医学院学报2024，Vol.30Issue(24)：1869-1875,7.DOI:10.13210/j.cnki.jhmu.20241022.001
欣胃颗粒调控METTL6/CyclinD1通路对胃癌细胞增殖、凋亡和细胞周期的影响
Effects of Xinwei granules on the METTL16/CyclinD1 pathway in gastric cancer cell proliferation,apoptosis,and cell cycle
摘要
Abstract
Objective:To investigate the effects of Xinwei granules on the METTL16/CyclinD1 pathway and proliferation,apoptosis,and cell cycle of gastric cancer cells,and to elucidate the molecular mechanisms underlying its therapeutic potential in gastric cancer.Methods:Cell viability of the MKN-45 gastric cancer cell line was assessed using the CCK-8 assay following treat-ment with various concentrations of gastric-derived granules.Flow cytometry was employed to analyze apoptosis and cell cycle dis-tribution.Western blot and RT-qPCR analyses were used to measure the expression of key molecules in the METTL16/CyclinD1 pathway.Spot hybridization was performed to detect m6A methylation modifications of CyclinD1.Results:Compared to the con-trol group,gastric-derived granules significantly increased apoptosis in MKN-45 cells,elevated the proportion of cells in the G1 phase,reduced the proportion in the S phase,and downregulated the expression of CyclinD1,CDK4,E2F1,and METTL16 proteins as well as the p-Rb/Rb ratio(P<0.01).Additionally,gastric-derived granules significantly decreased the mRNA expres-sion levels of METTL16 and CyclinD1,and inhibited CyclinD1 m6A methylation modifications(P<0.01).Conclusion:Xinwei granules exert their effects by inhibiting METTL16-mediated m6A methylation of CyclinD1,thereby blocking the transition from the G1 phase to the S phase,suppressing cell proliferation,and promoting apoptosis.关键词
欣胃颗粒/METTL16/CyclinD1信号通路/m6A甲基化修饰/胃癌前病变Key words
Xinwei granules/METTL16/CyclinD1 signaling pathway/m6A methylation modifications/Precancerous le-sions of gastric cancer分类
医药卫生引用本文复制引用
吴丽丽,张冉,刘馨瑶,张杨,王静滨..欣胃颗粒调控METTL6/CyclinD1通路对胃癌细胞增殖、凋亡和细胞周期的影响[J].海南医学院学报,2024,30(24):1869-1875,7.基金项目
This study was supported by the National Natural Science Foundation of China(81973601) （81973601）
Shenzhen Futian District Health System Research Project(FTWS2022033 and FTWS2023099) （FTWS2022033 and FTWS2023099）
Funding Support for the Construction of Clinical Key Specialties in Futian District,Shenzhen. 国家自然科学基金资助项目(81973601) （81973601）
深圳市福田区卫生健康系统科研项目(FTWS2022033) （FTWS2022033）
深圳市福田区卫生健康系统科研项目(FTWS2023099) （FTWS2023099）
深圳市福田区临床重点专科建设经费资助 （）
