海南医学院学报2024，Vol.30Issue(24)：1876-1886,11.DOI:10.13210/j.cnki.jhmu.20240903.001
天香丹调节线粒体能量代谢治疗心肌缺血损伤的作用机制研究
Research on the mechanism of Tianxiangdan regulating mitochondrial energy metabolism in treating myocardial ischemic injury
摘要
Abstract
Objective:To screen mitochondrial energy metabolism related genes in myocardial ischemic injury through bioinfor-matics,network pharmacology,immune infiltration analysis,and molecular docking techniques,and to reveal whether the mecha-nism of Tianxiangdan in treating myocardial ischemic injury is related to regulating mitochondrial energy metabolism.Methods:Differential expression genes(DEGs)of"Tianxiangdan myocardial ischemia-reperfusion injury mitochondrial energy metabolism"were screened through network pharmacology and bioinformatics,and their correlation was analyzed.Gene enrichment analysis was performed on mitochondrial energy metabolism DEGs,and a protein-protein interaction(PPI)network was constructed to screen for potential key(Hub)genes involved in mitochondrial energy metabolism in myocardial ischemia-reperfusion injury.By scoring the immune cells and immune function of the gene set,evaluate the level of immune cell infiltration,and perform immune infiltration analysis and gene set enrichment analysis(GSEA)on key genes separately.Subsequently,in order to investigate the re-lationship between mitochondrial energy metabolism DEGs and high-risk factors,a correlation analysis was conducted between mi-tochondrial energy metabolism DEGs and risk factors.Molecular docking technology was used to verify the interactions between important components and key molecular targets.Finally,animal experiments were conducted to further verify whether the key genes GNAI2,Mfn2 are the key molecular targets of Tianxiangdan in regulating mitochondrial energy metabolism for the treat-ment of myocardial ischemic injury.Results:After screening,a total of 24 differentially expressed genes were identified for"Tianx-iangdan myocardial ischemia-reperfusion injury mitochondrial energy metabolism".Correlation analysis revealed strong synergistic effects among key genes.The gene enrichment results revealed that key genes have the functions of mutual regulation,immune regulation,and mediating multiple signaling pathways.Ten Hub genes closely related to potential mitochondrial energy metabo-lism in myocardial ischemic injury were identified through PPI network screening,and these key genes showed strong correlations with immune processes and inflammatory responses in both immune infiltration analysis and GSEA enrichment analysis.Finally,the correlation analysis of risk factors for key genes confirmed the clinical predictive efficacy of the aforementioned genes for myo-cardial ischemic injury disease.Conclusion:The regulation of mitochondrial energy metabolism by Tianxiangdan in the treatment of myocardial ischemic injury may be closely related to key molecular targets such as GNAI2,Mfn2,as well as multiple signaling pathways and immune regulatory mechanisms mediated by TNF signaling pathway,cAMP signaling pathway,etc.关键词
天香丹/心肌缺血损伤/线粒体能量代谢/机制研究/实验验证Key words
Tianxiangdan/Myocardial ischemic injury/Mitochondrial energy metabolism/Mechanism research/Experimen-tal validation分类
医药卫生引用本文复制引用
郭闫闫,赵明芬,侯晓叶,袁茜茜..天香丹调节线粒体能量代谢治疗心肌缺血损伤的作用机制研究[J].海南医学院学报,2024,30(24):1876-1886,11.基金项目
This study was supported by 2024 Xinjiang Uygur Autonomous Region Graduate Innovation Project(XJ2024G162) （XJ2024G162）
National Natural Science Foundation of China(82160876) 2024年新疆维吾尔自治区研究生创新项目(XJ2024G162) （82160876）
国家自然科学基金资助项目(82160876) （82160876）
