广东医学2024，Vol.45Issue(12)：1515-1520,6.DOI:10.13820/j.cnki.gdyx.20231156
湿证模型的构建与验证
Construction and verification of dampness syndrome model
摘要
Abstract
Objective To establish the dampness syndrome model by intraperitoneal injection of low-dose li-popolysaccharide(LPS)in rats.Methods 30 male SD rats were randomly divided into normal group,LPS 600 μg/kg group and LPS 1 200 μg/kg group.The general condition,body weight,water intake and intake of the rats were assessed.Splenic macrophages and CD4+T cell subsets were detected.Serum biochemical,interleuhin(IL)-1 β,tumor necrosis factor-α(TNF-α)and γ-interferon(1FN-γ)levels were detected.HE staining was used to detect the pathological changes of liver,lung and kidney.The positive expression of CD86 in lung and kidney tissues was detected by immunohis-tochemistry.Results Compared with normal group,LPS group rats lost weight,overall posture was more sluggish,slow gait,slow response to stimulation,perianal filth.The levels of albumin and blood lipid were decreased(P＜0.05),M1 macrophages of spleen were activated(P＜0.01),and serum IL-1 β level was increased(P＜0.05).Liver,lung and kidney tissues showed certain damage,with infiltration of M1 macrophages in lung and kidney tissues.Conclusion This model can be regarded as a stable and reliable animal model of pathogenic factor wet syndrome.关键词
湿证/模型构建/生物致病因子/脂多糖Key words
dampness syndrome/model construction/biological pathogenic factor/lipopolysaccharide分类
医药卫生引用本文复制引用
卢钊宇,胡冬梅,黎创,黄丽华,裴鸿飞,黄燕,毛炜..湿证模型的构建与验证[J].广东医学,2024,45(12):1515-1520,6.基金项目
省部共建中医湿证国家重点实验室专项课题(SZ2021ZZ10) （SZ2021ZZ10）
