化工学报2024，Vol.75Issue(z1)：251-258,8.DOI:10.11949/0438-1157.20240534
谷胱甘肽双功能合成酶分子改造及应用
Molecular modification of glutathione bifunctional synthase and its application
摘要
Abstract
Glutathione(GSH)is the most abundant non-protein thiol within cells.It has strong electron donating ability and participates in redox reactions in organisms,which endows it with multiple physiological functions and it is widely used in food,pharmaceutical and cosmetics industries.Glutathione bifunctional synthase is the key enzyme for catalyzing the production of GSH and improvement of its catalytic efficiency is of significance to GSH biosynthesis.In this study,the glutathione bifunctional synthasefrom Streptococcus thermophilus(St-GshF)was chosen for study object.A high-throughput screening method based on the fluorescence colorimetric effect generated by the reaction between GSH and ortho benzaldehyde was established.Using semi-rational design,St-GshF was engineered and the mutant St-GshF(S27Q/G510P)was obtained.Its activity was 1.75 times higher than the wild type.The polyphosphate kinase was coupled to construct the efficient glutathione biosynthesis system and after optimization of the reaction conditions,17.36 g/L glutathione was obtained with a yield of 94.22％,laying an important foundation for the large-scale production of glutathione.关键词
谷胱甘肽双功能合成酶/谷胱甘肽/分子改造/双酶偶联Key words
glutathione bifunctional synthetase/glutathione/molecular modification/dual enzyme coupling分类
生物科学引用本文复制引用
宋世萍,汤晓玲,郑仁朝..谷胱甘肽双功能合成酶分子改造及应用[J].化工学报,2024,75(z1):251-258,8.基金项目
国家重点研发计划项目(2023YFA0914000) （2023YFA0914000）
