渔业科学进展2025，Vol.46Issue(1)：183-193,11.DOI:10.19663/j.issn2095-9869.20240402002
2022-2023年虾类传染性肌坏死病毒(IMNV)流行情况调查
Investigation of the Prevalence of Infectious Myonecrosis Virus(IMNV)from 2022 to 2023
摘要
Abstract
Infectious muscle necrosis(IMN)caused by infectious myonecrosis virus(IMNV)broke out for the first time in Chinese shrimp in 2020,causing substantial economical loss to the shrimp farming industry.To understand the prevalence of Chinese IMNV in recent years,an epidemiological survey of IMNV was conducted in major Chinese shrimp farming areas between 2022-2023.Samples were analyzed using molecular biology and histopathology.A total of 829 samples were collected from Shandong,Jiangsu,Zhejiang,Hainan,Tianjin,Guangxi,Fujian,and Hebei.These samples included major farmed shrimp species,such as Penaeus vannamei,P.japonicas,P.chinensis,and Macrobrachium rosenbergii,as well as live feed,other aquatic economic species,and culture water.TaqMan real-time quantitative fluorescence RT-PCR(TaqMan RT-qPCR)was used for molecular detection.The skeletal muscle of diseased shrimp exhibited the typical plaque or diffuse white necrosis symptom of IMNV infection.IMNV was detected in major farmed shrimp species such as P.vannamei,P.japonicus,and P.chinensis.The positive samples were mainly distributed in Shandong,Hebei,Tianjin,and other provinces or cities around the Bohai Sea.In addition to farmed shrimp,IMNV-positive shrimp live bait(mainly Artemia)and offshore seawater filtered close to the farm was also detected.The IMNV-positive rates of samples collected in 2022 and 2023 were 6.27％(23/367)and 15.80％(73/462),respectively.The IMNV-positive TaqMan RT-qPCR samples were further analyzed using histopathological and in situ hybridization methods.The characteristic coagulation necrosis of IMNV infection was found in the white muscle tissue sections of the ventral and caudal segments of the diseased shrimp,with a clear blue-violet hybridization signal of the IMNV probe in the muscle tissue with pathological damage.The results of this study indicated a high positive rate of IMNV in cultured shrimp,biological live bait,and offshore seawater in many Chinese provinces between 2022-2023.Detection,monitoring,and early warning of IMNV infection in the shrimp farming process should be strengthened to reduce the risk of further spread and IMN epidemics.关键词
传染性肌坏死病毒(IMNV)/流行病学/TaqManRT-qPCR/组织病理/组织原位杂交Key words
Infectious myonecrosis virus(IMNV)/Epidemiology/TaqMan RT-qPCR/Tissue histopathology/In situ hybridization分类
农业科技引用本文复制引用
徐瑞东,夏继涛,李萍,余星潼,姚亮,李文强,贾田畅,张庆利..2022-2023年虾类传染性肌坏死病毒(IMNV)流行情况调查[J].渔业科学进展,2025,46(1):183-193,11.基金项目
中国水产科学研究院基本科研业务费(2020TD39 （）
2021XT0602 （）
2023TD42)、中国水产科学研究院黄海水产研究所级基本科研业务费(20603022022024)和国家虾蟹产业技术体系(CARS-48)共同资助. （20603022022024）
