吉林农业大学学报2024，Vol.46Issue(6)：962-970,9.DOI:10.13327/j.jjlau.2020.5838
对松材线虫胁迫响应的哈茨木霉细胞色素P450家族基因的生物信息学分析
Bioinformatics Analysis of Trichoderma harzianum Cytochrome P450 Family Responding to Biological Stress Test of Bursaphelenchus xylophilus
摘要
Abstract
Ten cytochrome P450(ThCYP)genes were selected from transcriptome data of Tricho-derma harzianum E15 under the stress test of Bursaphelenchus xylophilus,which were named ThCYP1～ThCYP10 in numerical order.The T.harzianum E15 ThCYP gene was studied by bioinfor-matics methods,such as gene physicochemical properties,phylogenetic relationship,amino acid se-quence alignment,gene structure analysis,conservative motif analysis and promoter analysis.These 10 ThCYP genes were basically similar in physicochemical properties,and the subcellular localiza-tion was observed mainly on the mitochondrial membrane.By establishing a phylogenetic tree of the CYP genes of Trichoderma virens and Trichoderma reesei and the E15 ThCYP gene of T.harzianum,it was found that the genetic relationship is very close.In amino acid multiple-sequences align-ments,these 10 ThCYP genes have multiple conserved sites to form conserved domains,including oxygen-binding domains,K-helix sequences,and conserved structural sequences of heme binding sites.In terms of gene structure,it was found that CDS regions are complete and the gene lengths are basically similar.Analysis of conserved motifs shows that the conserved motifs of the ten ThCYP genes are remarkably similar and share most of the same conserved motifs to play a key role in stress resistance.In the analysis of the promoter region,seven homeopathic components from the ten ThCYP genes are distributed in the promoter region,mainly ABRE and CGTCA-motifs.These prop-erties provide a remarkable contribution for resisting against B.xylophilus,where ThCYP2 has TC-rich repeats distributed in the promoter region,further increasing the resistance against B.xylophilus.关键词
哈茨木霉E15/松材线虫/CYP基因/生物信息学分析Key words
Trichoderma harzianum E15/Bursaphelenchus xylophilus/CYP gene/bioinformatics analysis分类
农业科技引用本文复制引用
张锴,逯昕明,周贺,焦宁,赫俊阳,宋瑞清..对松材线虫胁迫响应的哈茨木霉细胞色素P450家族基因的生物信息学分析[J].吉林农业大学学报,2024,46(6):962-970,9.基金项目
国家林业局"948"项目(2015-4-34) （2015-4-34）
