吉林农业大学学报2024，Vol.46Issue(6)：979-987,9.DOI:10.13327/j.jjlau.2021.1920
2种灵芝原生质体制备及再生条件优化
Optimization of Preparation and Regeneration Conditions of Two Kinds of Ganoderma lucidum Protoplasts
摘要
Abstract
Using Ganoderma lingzhi and Ganoderma sessile strains as experimental materials,the ef-fects of mycelial age,enzymolysis time,enzymolysis temperature and centrifugal speed on the prepa-ration of Ganoderma lucidum protoplasts were compared and analyzed through single factor test and orthogonal test,the preparation conditions were optimized,and the effects of different types and con-centrations of osmotic stabilizers and different culture methods on the regeneration of Ganoderma lu-cidum protoplasts were preliminarily discussed.The results showed that the optimal conditions for the preparation of Ganoderma lingzhi protoplasts were mycelial age of 10 days,enzymolysis tem-perature of 35℃,enzymolysis for 3 hours,centrifugation at 4 000 r/min,and protoplast yield of 9.75×107/mL;Ganoderma sessile protoplasts were prepared at a mycelial age of 7 days,enzymolysis temperature of 31℃,enzymolysis for 3.5 hours,centrifugation at 3 000 r/min,and a protoplast yield of 7.48×107/mL.Ganoderma lucidum strains were regenerated with 0.5 mol/L mannitol as osmotic sta-bilizer,and the double-layer plate culture method(with an upper agar concentration of 1.1%)could obtain a high regeneration rate.关键词
灵芝/原生质体/正交试验/再生率Key words
Ganoderma lucidum/protoplast/orthogonal test/regeneration rate分类
农业科技引用本文复制引用
许琳,刘洋,张雪超,胡清秀,闫梅霞..2种灵芝原生质体制备及再生条件优化[J].吉林农业大学学报,2024,46(6):979-987,9.基金项目
吉林省发展和改革委员会项目(20220038-5),天津市合成生物技术创新能力提升行动项目(TSBICIP-CXRC-006) （20220038-5）
