吉林农业大学学报2024，Vol.46Issue(6)：988-994,7.DOI:10.13327/j.jjlau.2021.1500
毛头鬼伞菌盖中蛋白质提取条件优化及氨基酸分析
Optimization of Protein Extraction from Pileus of Coprinus comatus and Analysis of Its Amino Acid
摘要
Abstract
Ultrasonic assisted sodium hydroxide alkaline method was used to extract protein from pi-leus of Coprinus comatus.Through single factor test and orthogonal test,the effects of solid-liquid ra-tio,temperature,time,ultrasonic power,and pH value on protein extraction rate were analyzed,and the optimal extraction process was determined.The composition of amino acids was analyzed by pre-column derivatized high performance liquid chromatography,and the nutritional value of amino acids was preliminarily evaluated by amino acid ratio coefficient score(SRC)and chemical score(CS).The optimal extraction conditions were as follows:pH 11,50℃,solid-liquid ratio 1∶40,60 min,and ul-trasonic power 200 W.Under these conditions,the extraction rate was 33.18%.There are a variety of amino acids in the pileus of Coprinus comatus.The essential amino acids accounted for 35.9%of the total amino acids,and EAA/NEAA was 0.58,which is close to the WHO recommended value.关键词
毛头鬼伞/菌盖/蛋白质提取/氨基酸Key words
Coprinus comatus/pileus/protein extraction/amino acid分类
农业科技引用本文复制引用
苏玉春,俞佳宝,李文君,汪树生,向应欣..毛头鬼伞菌盖中蛋白质提取条件优化及氨基酸分析[J].吉林农业大学学报,2024,46(6):988-994,7.基金项目
吉林省自然科学基金项目(20170101108JC),吉林省大学生创新创业大赛项目(2021) （20170101108JC）
