江西农业学报2024，Vol.36Issue(12)：73-79,7.DOI:10.19386/j.cnki.jxnyxb.2024.12.010
超高效液相色谱串联三重四级杆质谱分析方法检测猪肉中多种药物残留
UPLC-MS/MS Method for Determination of Multiple Drug Residues in Pork
张苏珍 1贺燕 1陶威 1田蕴 1葛敏 1王益军1
作者信息
- 1. 连云港市畜产品质量监督检验测试中心,江苏 连云港 222000
- 折叠
摘要
Abstract
The samples were extracted with 80%acetonitrile solution,purified with PRiME HLB solid phase extraction column,concentrated with nitrogen,and finally dissolved with 10%methanol.A gradient elution was performed using 0.01%formic acid water-methanol as a mobile phase for gradient elution.The multi-reaction monitoring(MRM)mode of UPLC-MS/MS was used for the determination,and quantification was performed by internal standard method.The results showed that the concentration of 30 compounds showed good linearity in the range of 0.5～20.0 ng/mL,with the coefficient correlation(R2)between 0.993997 to 0.999872.At the addition levels of 1.0,5.0 and 10.0 μg/kg,the recovery ratio of 30 compounds was 85.9%～115.8%in pork.The detection limit of 30 compounds was 0.02～1.00 μg/kg,and quantification limit was 0.06～3.00 μg/kg.The test indicated that the method was simple,sensitive,accurate,stable in recovery,good accuracy and repeatability,and suitable for the detection and analysis of large quantities of pork samples.关键词
UPLC-MS/MS/猪肉/药物/残留/梯度洗脱/内标法Key words
UPLC-MS/MS/Pork/Drug/Residues/Gradient elution/Internal standard method分类
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张苏珍,贺燕,陶威,田蕴,葛敏,王益军..超高效液相色谱串联三重四级杆质谱分析方法检测猪肉中多种药物残留[J].江西农业学报,2024,36(12):73-79,7.
