超高效液相色谱串联三重四级杆质谱分析方法检测猪肉中多种药物残留OACSTPCD

The samples were extracted with 80%acetonitrile solution,purified with PRiME HLB solid phase extraction column,concentrated with nitrogen,and finally dissolved with 10%methanol.A gradient elution was performed using 0.01%formic acid water-methanol as a mobile phase for gradient elution.The multi-reaction monitoring(MRM)mode of UPLC-MS/MS was used for the determination,and quantification was performed by internal standard method.The results showed that the concentration of 30 compounds showed good linearity in the range of 0.5～20.0 ng/mL,with the coefficient correlation(R2)between 0.993997 to 0.999872.At the addition levels of 1.0,5.0 and 10.0 μg/kg,the recovery ratio of 30 compounds was 85.9%～115.8%in pork.The detection limit of 30 compounds was 0.02～1.00 μg/kg,and quantification limit was 0.06～3.00 μg/kg.The test indicated that the method was simple,sensitive,accurate,stable in recovery,good accuracy and repeatability,and suitable for the detection and analysis of large quantities of pork samples.