基础医学与临床2025，Vol.45Issue(1)：7-11,5.DOI:10.16352/j.issn.1001-6325.2025.01.0007
褪黑素促进小鼠黑色素瘤细胞系B16-F10失巢凋亡
Melatonin promotes anoikis of mouse melanoma cell line B16-F10
摘要
Abstract
Objective To investigate the effect and mechanism of melatonin on the anoikis of melanoma cells.Methods The drug concentration of melatonin inhibiting melanoma cell line B16-F10 was optimized based on the effect on CCK-8 assay.An anti-anoikis of melanoma cell model was developed and divided it into four groups:The blank control group,the TrkB activator group,the melatonin group and the melatonin+TrkB activator group.Calce-in AM/EthD-1 fluorescence double staining was used to detect the anoikis of melanoma cells.Reactive oxygen spe-cies were detected using the fluorescent probe DCFH-DA.Western blot was used to detect the expression of Nrf2 protein and TrkB protein in each group.Results Melatonin significantly inhibited the proliferation of melanoma cells in a time-and dose-dependent manner with IC50 of 1×10-7 μmol/L.Its inhibitory effect was found to be related to in-duction of anoikis of melanoma cells.Melatonin could upregulate the generation of cellular reactive oxygen species(P<0.05),while addition of TrkB activator antagonized this effect.Melatonin could reduce the expression of Nrf2 protein and TrkB protein in melanoma cells(P<0.05),and the addition of TrkB activator could inhibite the effect of melatonin on the expression of Nrf2 protein and TrkB protein(P<0.05).Conclusions Melatonin can inhibit the pro-liferation of melanoma cell line B16-F10 through the mechanism of inducing anoikis.关键词
黑色素瘤/褪黑素/失巢凋亡/活性氧Key words
melanoma/melatonin/anoikis/reactive oxygen species分类
临床医学引用本文复制引用
甘雨灵,李廷栋,刘利兵,周英芬,潘东升..褪黑素促进小鼠黑色素瘤细胞系B16-F10失巢凋亡[J].基础医学与临床,2025,45(1):7-11,5.基金项目
甘肃省自然科学基金(22JR5RA645) （22JR5RA645）
兰州市科技计划项目(2023-ZD-53) （2023-ZD-53）
