基础医学与临床2025，Vol.45Issue(1)：20-24,5.DOI:10.16352/j.issn.1001-6325.2025.01.0020
下调CD151联合贝伐珠单抗抑制结直肠癌生长与微血管密度
Down-regulation of CD151 combined with bevacizumab inhibits the growth and microvessel density of colorectal cancer
摘要
Abstract
Objective To investigate the effects of CD151 down-regulation combined with bevacizumab on colorectal cancer growth and microvessel density.Methods Human colorectal cancer cell line HT-29 and CD151--HT-29 cells strain(CD151 down-regulated HT-29 cells)were treated with bevacizumab.The cells were divided into four groups:control(HT-29)group,bevacizumab-treatment group,CD151--HT-29 group,and CD151--HT-29+bevacizumab-treatment group.Cell proliferation was observed in each group using the MTS assay.A subcutaneous xenograft model in nude mice was established,and the HT-29 control group and CD151--HT-29 group were treated with either 0.9％NaCl solution or bevacizumab.The growth of subcutaneous tumors in the four groups was ob-served,and the volume and weight of the tumors were recorded.Tumor tissues were collected for immunohistochem-ical staining of endothelial cells to assess microvessel density(MVD).Results Compared with the control group,cell proliferation was significantly reduced in the bevacizumab-treated group and CD151--HT-29 group(P<0.001).Cell proliferation in the CD151--HT-29+bevacizumab-treated group was slower than that in the single treatment groups(P<0.001).In the subcutaneous tumor model,the volume,weight,and MVD of tumors in the bevacizumab-treated and CD151--HT-29 groups were significantly reduced compared to the control group(P<0.01).In the CD151--HT-29+bevacizumab group,the tumor volume,weight,and CD34 expression were significantly lower than in the single treatment groups(P<0.01).Conclusions CD151 protein may play a role in the regulation of angiogenesis in colorectal cancer tissues and may have a synergistic effect with bevacizumab in in-hibiting microvessel formation in tumor tissues.关键词
结直肠癌/CD151/贝伐珠单抗/微血管密度Key words
colorectal cancer/CD151/bevacizumab/microvessel density分类
中医学引用本文复制引用
刘艳彩,刘学刚,张振亚..下调CD151联合贝伐珠单抗抑制结直肠癌生长与微血管密度[J].基础医学与临床,2025,45(1):20-24,5.基金项目
衡水市科技计划项目(2016014054Z) （2016014054Z）
河北省科技计划项目(13397708D) （13397708D）
