基础医学与临床2025，Vol.45Issue(1)：25-30,6.DOI:10.16352/j.issn.1001-6325.2025.01.0025
赤芍总苷减轻脑缺血/再灌注模型大鼠脑损伤
Total paeony glycoside alleviates brain injury of rat models developed by cerebral ischemia-reperfusion
摘要
Abstract
Objective To investigate the effect of total paeony glycoside(TPG)on cerebral ischemia-reperfusion injury(CI/RI)of rats.Methods The rats were randomly divided into sham surgery(sham)group,CI/RI model group(simple CI/RI group),positive control group(nimodipine group,5 mg/kg),low-dose TPG group(TPG-L group,27 mg/kg),a high-dose TPG group(TPG-H group,54 mg/kg)and a high-dose TPG+NOD-like receptor thermal protein domain associated protein 3(NLRP3)activator diethyl dithiocarbamate(DDC)group(TPG-H+DDC group,54 mg/kg TPG and 30 mg/kg DDC),with 18 rats in each,administered once a day for 7 consecutive days.After the administration,the neurological deficit score of the rats was evaluated.Nissl staining microscopy was applied to observe neuronal activity in brain tissue.2,3,5-triphenyltetrazolium chloride(TTC)staining microscopy was applied to detect the area of cerebral infarction in rats.The level of interleukin-1β and IL-18 in brain tissue was measured by ELISA method.Western blot was applied to detect the expression of purinergic receptor P2X ligand-gated ion channel 7(P2X7R)/NLRP3 signaling pathway related proteins and pyroptosis related proteins such as apoptosis associated speck like protein containing a CARD(ASC)and cysteine protease 1(caspase-1)proteins in brain tissue.Results Compared with the sham group,the neurological deficit score,infarct area,level of IL-1β and IL-18 in brain tissue and protein expression of P2X7R,NLRP3,ASC,and caspase-1 in brain tissue of rats in the simple CI/RI group were significantly increased(P<0.05),while the proportion of Nissl body positive cells in brain tissue was significantly reduced(P<0.05).The change in corresponding indicators of rats in the nimodipine group,TPG-L group,and TPG-H group was opposite to those in the simple CI/RI group(P<0.05).NLRP3 acti-vator DDC antagonized the inhibitory effect of TPG on cell pyroptosis in CI/RI rats.Conclusions TPG may inhibit brain injury in CI/RI rats by down-regulating the P2X7R/NLRP3 pathway.关键词
赤芍总苷/P2X7R/NLRP3通路/脑缺血/再灌注损伤/焦亡Key words
total paeony glycoside/P2X7R/NLRP3 pathway/cerebral ischemia-reperfusion injury/pyroptosis分类
临床医学引用本文复制引用
彭莹娟,李志营,孙林林,杨会杰,王甜甜,周丽平..赤芍总苷减轻脑缺血/再灌注模型大鼠脑损伤[J].基础医学与临床,2025,45(1):25-30,6.基金项目
河南省医学科技攻关计划联合共建项目(LHGJ20230741) （LHGJ20230741）
