基础医学与临床2025，Vol.45Issue(1)：38-43,6.DOI:10.16352/j.issn.1001-6325.2025.01.0038
姜黄素对嗜铬细胞瘤细胞系PC12增殖及侵袭的影响
Effects of curcumin on the proliferation and invasiveness of pheochromocytoma cell line PC12
摘要
Abstract
Objective To investigate the effects of curcumin on the proliferation,migration,invasion,and apoptosis of pheochromocytomacell line PC12.Methods PC12 cells were incubated with different concentrations of curcumin.Cell proliferation was assessed using the CCK-8 assay to determine the IC50.The scratch assay was used to evaluate cell migration and Transwell chambers were employed to assess cell invasiveness.Flow cytometry was used to analyze apoptosis.qPCR was conducted to measure the mRNA expression of pro-apoptotic(Bax)and anti-apoptotic(Bcl-2)genes,and Western blot was performed to detect Bax and Bcl-2 protein expressions.Results Curcumin(10-80 μmol/L)inhibited PC12 cell proliferation in a concentration-dependent manner,with an IC50 as 29 μmol/L.Curcumin also suppressed PC12 cell migration in a concentration-dependent mode;the migration rate decreased from 66％in the control group down to 51％,5％,and 0.5％in the 10,20,and 30 μmol/L curcumin groups,respectively.Curcumin at concentrations of 20-30 μmol/L significantly reduced PC12 cell invasiveness(P<0.000 1).Moreover,curcumin significantly promoted PC12 cell apoptosis;the percentage of apoptotic cells increased by 2.25％,18.53％,and 26.89％in the 10,20,and 30 μmol/L curcumin groups as compared to those of control group,respectively.Curcumin treatment resulted in a significant up-regulation of Bax mRNA and protein expression,and a significant down-regulation of Bcl-2 mRNA and protein expression(P<0.05).Conclusions Curcumin may significantly inhibit the proliferation,migration,and invasion of PC12 cells and arouse cell apopto-sis.Its pro-apoptotic effect may be associated with alterations in the expression of Bax and Bcl-2 genes.关键词
姜黄素/PC12细胞/凋亡/侵袭/迁移Key words
curcumin/PC12 cell/apoptosis/invasion/migration分类
临床医学引用本文复制引用
张文倩,周玥,任卫东,童安莉..姜黄素对嗜铬细胞瘤细胞系PC12增殖及侵袭的影响[J].基础医学与临床,2025,45(1):38-43,6.基金项目
国家重点研发计划(2021YFC2501600,2021YFC2501603) （2021YFC2501600,2021YFC2501603）
中央高水平医院临床研究专项(2022-PUMCH-C-028) （2022-PUMCH-C-028）
中国医学科学院医学与健康科技创新工程(2021-I2M-C&T-B-002,2021-I2M-1-007) （2021-I2M-C&T-B-002,2021-I2M-1-007）
