口腔颌面外科杂志2024，Vol.34Issue(6)：427-434,8.DOI:10.12439/kqhm.1005-4979.2024.06.003
lincRNA-EPS/miR-24-3p/BMP2信号轴对牙釉质发育影响的体外实验研究
Effect of lincRNA-EPS/miR-24-3p/BMP2 signal axis on enamel development:An experimental study in vitro
摘要
Abstract
Objective:To investigate the effect of long intergenic non-coding RNA-EPS(lincRNA-EPS)/microRNA(miRNA)-24-3p/bone morphogenetic protein 2(BMP2)signal axis on enamel development in vitro.Methods:The overexpressed plasmid of lincRNA-EPS and miR-24-3p mimics were transfected into LS8 cells and their transfection efficiency was detected by real-time quantitative polymerase chain reaction(RT-qPCR).RT-qPCR was used to detect the relative mRNA expression of amelogenesis-related genes(AMELX,AMBN,AMTN,MMP20)and BMP2 after transfection.Western blotting was used to detect the protein expression of BMP2 after transfection.Independent sample t test was used for quantitative comparison between groups.Results:Fluorescence images under microscope and RT-qPCR results showed that lincRNA-EPS overexpression plasmid and miR-24-3p mimics were transfected successfully.The relative mRNA expression of amelogenesis-related genes and BMP2 as well as the protein expression of BMP2 were significantly increased after transfection with lincRNA-EPS overexpression plasmid,while the relative mRNA expression of amelogenesis-related genes and BMP2 as well as the protein expression of BMP2 were significantly decreased after transfection with miR-24-3p mimics.To a certain extent,lincRNA-EPS can inhibit the regulatory effect of miR-24-3p on BMP2,and the difference was statistically significant(P<0.05).Conclusion:Enamel development is regulated by lincRNA-EPS/miR-24-3p/BMP2 signal axis,and lincRNA-EPS can affect the inhibition of miR-24-3p on downstream BMP2 expression,thus promoting enamel development.关键词
长链基因间非编码RNA-EPS/微小RNA-24-3p/骨形态发生蛋白2/牙釉质发育Key words
long intergenic noncoding RNA-EPS/microRNA-24-3p/bone morphogenetic protein 2/enamel development分类
医药卫生引用本文复制引用
俞秀君,苏俭生..lincRNA-EPS/miR-24-3p/BMP2信号轴对牙釉质发育影响的体外实验研究[J].口腔颌面外科杂志,2024,34(6):427-434,8.基金项目
国家自然科学基金(82170913) （82170913）
上海市科学技术委员会项目(201409006200) （201409006200）
