林业科学2024，Vol.60Issue(12)：58-71,14.DOI:10.11707/j.1001-7488.LYKX20230648
外源茉莉酸甲酯对红椿苗木干旱损伤的缓解作用及生理机制
Alleviation Effect and Physiological Mechanism of Exogenous Methyl Jasmonate on Drought Damage of Toona ciliata Seedlings
摘要
Abstract
[Objective]To explore the alleviation effect of exogenous spraying methyl jasmonate(MeJA)on drought damage of T.ciliata seedlings and its physiological mechanism,and to determine the key regulatory factors of MeJA to improve the drought tolerance of T.ciliata.[Method]In this study,2-year-old T.ciliata seedlings were used as materials,and pot water control experiments were conducted to explore the mitigation effects of MeJA on the growth,osmotic regulation,antioxidant and photosynthetic physiological processes of T.ciliata under moderate drought stress by spraying MeJA solution on the leaves.[Result]MeJA could alleviate the growth inhibition of drought on T.ciliata seedlings,restore cell morphology,and increase plant height and leaf growth rate.MeJA could promote the accumulation of soluble protein(SP)and starch(St)in T.ciliata leaves and restore cell osmotic potential.MeJA could also enhance the scavenging ability of reactive oxygen species(ROS)by enhancing the efficiency of AsA-GSH cycle in leaves,reduce the contents of malondialdehyde(MDA),superoxide anion(O2-·)and hydrogen peroxide(H2O2),reduce oxidative damage,protect cell structure and function,and improve the drought resistance of T.ciliata seedlings.In addition,exogenous MeJA could inhibit the decomposition of chlorophyll in T.ciliata leaves under drought stress,increase stomatal conductance(gs)and maintain its photosynthetic capacity.The VIP analysis of the PLS model further showed that SP was the key control factor affecting the drought resistance of T.ciliata,followed by O2-·,gs and transpiration rate(Tr).[Conclusion]Exogenous MeJA can effectively alleviate the damage of drought stress to T.ciliata seedlings by improving the osmotic adjustment ability and antioxidant capacity of T.ciliata leaves under drought stress,increasing stomatal conductance and improving photosynthesis.The results provide a new idea for the development of drought-resistant regulators and drought-resistant cultivation of T.ciliata.关键词
红椿/茉莉酸甲酯/干旱胁迫/光合特性/生理特性Key words
Toona ciliata/methyl jasmonate/drought stress/photosynthetic characteristics/physiological characteristics分类
农业科技引用本文复制引用
周凡博,刘玉民,刘亚敏,代崇雯,高琦,张钰林,朱娅婷..外源茉莉酸甲酯对红椿苗木干旱损伤的缓解作用及生理机制[J].林业科学,2024,60(12):58-71,14.基金项目
重庆市林业科技攻关项目(ylky2018-05) （ylky2018-05）
中央林业改革发展资金科技推广项目(ylkt2019-04). （ylkt2019-04）
