林业科学2024，Vol.60Issue(12)：101-110,10.DOI:10.11707/j.1001-7488.LYKX20230653
猕猴桃溃疡病生防菌群的构建及其生防效应
Construction and Control Effect of Biocontrol Flora of Kiwifruit Bacterial Canker
摘要
Abstract
[Objective]In this study,biocontrol bacteria were isolated from kiwifruit and used for construction of biocontrol bacterial community.The pathogen of kiwifruit bacterial canker was identified in Lin'an,Zhejiang Province.This study aims to explore the biocontrol effect of the bacterial community on the pathogen of kiwifruit bacterial canker,so as to provide new insights for the prevention and control of kiwifruit bacterial canker.[Method]The pathogenic bacteria causing canker disease of kiwifruit were isolated from kiwifruit planting areas in Lin'an,Zhejiang Province and identified.Based on the comparison and analysis of the metagenomes of endophytic bacteria in healthy and diseased kiwifruit branches using R language,antagonistic bacteria against the pathogenic bacteria were isolated,and a biocontrol bacterial community was constructed.The biocontrol effect of the bacterial community on the pathogenic bacteria was validated through in vitro co-cultivation and in vivo inoculation experiments.[Result]1)Kiwifruit bacterial canker in Lin'an,Zhejiang Province was caused by Pseudomonas syringae pv.actinidiae HY-05.The metagenomic sequencing results of endophytic bacteria in kiwifruit branches showed that positive interactions were more likely to form among the bacterial communities in healthy kiwifruit branches compared to diseased ones.2)Based on R language analysis,antagonistic strains,Kineococcus mangrovi ZC-3 and Enterococcus mundtii ZC-5,against the pathogenic strain HY-05,as well as a probiotic strain,Stenotrophomonas rhizophila ZC-6,were isolated from healthy kiwifruit plants,and a biocontrol bacterial community was constructed.3)In the in vitro co-cultivation experiment,all tested strains inhibited the growth of HY-05,with the biocontrol bacterial community constructed by ZC-3,ZC-5,and ZC-6 showing the best inhibitory effect,with a suppression rate of 100％.In the in vivo plant experiment,treatments with different biocontrol bacterial combinations were able to inhibit the occurrence of kiwifruit bacterial canker to varying degrees,with the biocontrol bacterial community constructed by ZC-3,ZC-5,and ZC-6 showing the best preventive effect,with a control efficiency of 56.00％,which was significantly different from the diseased control(P＜0.01).[Conclusion]Kiwifruit bacterial canker in Lin'an,Zhejiang Province is caused by P.syringae pv.actinidiae.The biocontrol bacterial community constructed from beneficial bacteria isolated from kiwifruit plants is proven to have practical biocontrol effects through in vitro and in vivo experiments.This study enriches the research system of biological control of bacterial canker in kiwifruit from a theoretical perspective,and the biocontrol bacterial community obtained can be used for precise prevention and control of kiwifruit bacterial canker,which is of practical significance for the development of the kiwifruit industry.关键词
猕猴桃溃疡病/宏基因组测序/R语言/生防菌/菌群构建/生防效应Key words
kiwifruit bacterial canker/metagenomic sequencing/R language/biocontrol bacteria/bacterial community construction/biocontrol effect分类
农业科技引用本文复制引用
尹志诚,顾佳颖,李南羿,张昕..猕猴桃溃疡病生防菌群的构建及其生防效应[J].林业科学,2024,60(12):101-110,10.基金项目
国家自然科学基金项目(31970093). （31970093）
