昆明医科大学学报2024，Vol.45Issue(12)：19-27,9.DOI:10.12259/j.issn.2095-610X.S20241203
藏红花素通过IRF7/NF-κB信号通路对垂体腺瘤的抑制作用
Inhibitory Effect of Crocin on Pituitary Adenomas via IRF7/NF-κB Signaling Pathway
摘要
Abstract
Objective To explore the role and mechanism of crocin in pituitary adenoma(PA)through clinical samples and related molecular biology experiments of HP75 cells.Methods From June 2022 to May 2023,16 PA samples were collected from the Second Department of Neurology and Otolaryngology skull base surgery of the First Affiliated Hospital of Kunming Medical University.Three normal control samples were from the human anatomy of the Forensic College of Kunming Medical University.The expression of IRF7 mRNA in clinical samples was detected,and the proliferation,migration,invasion and apoptosis of HP75 cells were detected by knocking down the expression of IRF7;the expression of NF-κB was regulated by IRF7 in HP75 cells,and crocin regulated the growth of PA cells and its regulatory effect on IRF7/NF-κB signaling pathway.Results RT-qPCR and immunohisto-chemistry showed that compared with the normal control group,the expression of IRF7 mRNA in PA was significantly increased(P＜0.001);the expression of IRF7 protein in si-IRF7 group was significantly decreased(P＜0.001);CCK-8,Transwell and flow cytometry results showed that compared with the control group,knockdown of IRF7 significantly decreased the cell viability of HP75 cells(P＜0.001),inhibited the migration and invasion(P＜0.001),and promoted the apoptosis of HP75 cells(P＜0.001).In addition,knockdown of IRF7 could inhibit the expression of p-NF-κB p65/NF-κB p65(P＜0.001)and p-NF-κB p65/NF-κB p65(P＜0.001).Overexpression of IRF7 partially reversed the effect of crocin(P＜0.001)and restored the expression of p-NF-κB p65/NF-κB p65(P＜0.01).Finally,the biological behavior of HP75 cells showed that compared with crocin group,overexpression of IRF7 could improve the cell viability of HP75 cells,promote their migration and invasion,and inhibit cell apoptosis(P＜0.001).Conclusion Crocin treatment can inhibit the proliferation,migration and invasion of PA cells,promote cell apoptosis,and alleviate the development of PA.In the mechanism,IRF7 is significantly overexpressed in PA,and knockdown of IRF7 can inhibit the malignant growth of PA.Crocin can inhibit the proliferation,migration and invasion of PA cells,and promote apoptosis by inhibiting IRF7/NF-κB signaling pathway.关键词
垂体腺瘤/藏红花素/IRF7/NF-κB信号通路/迁移/侵袭/细胞凋亡Key words
Pituitary adenoma/Crocetin/IRF7/NF-κB signaling pathway/Migration/Invasion/Apoptosis分类
医药卫生引用本文复制引用
刘卓慧,覃诗茵,赵鹤翔,贾峰峰,阮标,龙瑞清..藏红花素通过IRF7/NF-κB信号通路对垂体腺瘤的抑制作用[J].昆明医科大学学报,2024,45(12):19-27,9.基金项目
国家自然科学基金地区科学基金资助项目(82160513) （82160513）
云南省科技厅-昆明医科大学联合专项(202101AY070001-082) （202101AY070001-082）
云南省建设面向南亚东南亚科技创新中心专项(202303AP140005) （202303AP140005）
