江西农业学报2024，Vol.36Issue(12)：31-39,9.DOI:10.19386/j.cnki.jxnyxb.2024.12.005
海金沙幼孢子体生根壮苗条件的优化研究
Optimization of Rooting and Seedling Growth Conditions for Young Sporophytes of Lygodium japonicum
摘要
Abstract
In order to screen the optimal culture conditions for young sporophyte rooting and seedling growth of Lygodium japonicum,and obtain high-quality seedlings,young sporophytes from aseptic germination culture were used as the explants,and the effects of different culture densities(1,3,5 and 7 sporophytes/flask),different concentrations of inorganic salt in culture medium(1/4MS,1/2MS and MS),also sucrose(1%,2%and 3%of concentrations),exogenous hormones NAA and IBA(0.1,0.3 and 0.5 mg/L used singly or in pairs on rooting and seedling growth of young sporophytes were investigated.The results showed that the culture density of 5～7 sporophytes per flask was more suitable,and the young sporophytes grew well.The optimal culture medium for sporophyte rooting and seedling growth was 1/4MS+1%sucrose+0.3 mg/L IBA+0.3 mg/L NAA,which could significantly improve the fresh and dry weight of sporophytes.Transplanting should be carried out after cultivating for about 90 days,while the sporophyte leaves are larger and deeper green,and the root system is good.It should not be too late,otherwise sporophyte would be under nutrition-deficient,and the leaves would turn yellow and wilt.关键词
海金沙/孢子体/无菌培养/生根壮苗Key words
Lygodium japonicum/Sporophyte/Aseptic culture/Rooting and seedling growth分类
生物科学引用本文复制引用
刘薇,童家赟,詹若挺,刘赛..海金沙幼孢子体生根壮苗条件的优化研究[J].江西农业学报,2024,36(12):31-39,9.基金项目
广东省省级乡村振兴战略专项资金种业振兴项目(2022-NJS-00-002、粤财农[2022]184号) （2022-NJS-00-002、粤财农[2022]184号）
东莞市科技特派员项目. （）
