毛竹PeACO基因家族鉴定及箨鞘剥除后的表达分析OA北大核心

1-aminocyclopropane-1-carboxylate oxidase(ACO)is one of the key rate limiting enzymes in the ethylene synthesis process.In this study,genome-wide identification and expression analysis of the PeACO gene family were performed in order to investigate the effects of PeACOs on the lignification process of moso bamboo(Phyllostachys edulis)stalks.In this study,11 sequences of PeACOs with complete 2OG-FeⅡ_Oxy structural domains were identified in the moso bamboo genome by sequence comparison with ACO genes in rice(Oryza sativa);Physicochemical analysis of the proteins showed that these genes encoded 304～445 amino acids,and the molecular weights of the proteins ranged from 34 105.83 to 49 417.78 D,evolutionary relationship and gene structure analyses showed that pairs of genes in the same branch were highly conserved at the gene structure and protein levels.The collinearity analysis showed that ACO gene family had 6 co-linear gene pairs in moso bamboo,and suggested that genome replication events had an important effect on the expansion of the number of PeACOs genes.PeACOs were expressed in all tissues of moso bamboo,and the highest abundance was especially found in shoots during the rapid growth period.The removal of bamboo clum sheath during the rapid growth period led to the deepening of lignification of the culm.And the expression analysis showed that most PeACOs genes were significantly upregulated by the removal of bamboo sheaths,and indicated that these genes played an important role in this process,and the gene pairs with similar structures have similar expression patterns.This study provides theoretical basis for further study on the function of ACO gene family of bamboo.