基于SSR标记的澜沧江流域大理茶群体遗传多样性研究OA北大核心

Camellia taliensis is an important wild relative species of tea and an important part of tea resources.It is very important to study the genetic diversity and genetic relationship of Camellia taliensis population for better scientific protection and rational utilization of Camellia taliensis germplasm resources.Using the SSR marker technology,the genetic diversity and genetic relation-ships of 45 Camellia taliensis variations and 5 Camellia sinensis var.assamica varieties in 5 cities in the Lancang River Basin were determined and evaluated.The results showed that a total of 55 polymorphic sites were detected by 15 pairs of effective primers,with an average of 3.67 sites amplified by each pair of primers.The average expected heterozygosity(He)of Camellia taliensis resources was 0.346.The polymorphism information content(PIC)ranged from 0.024～0.661,with an average of 0.381.Shannon index ranged from 0.067～1.321,with an average of 0.669.The proportion of polymorphic primers was 60.0％,and the proportion of polymorphic primers to effective primers was 75.0％.The results of cluster analysis showed that the classification of C.taliensis germplasm in the Lancang River Basin was correlated with geographical regions.The genetic distance between C.taliensis populations with distant geographical locations was far,but the genetic distance within populations was close.The findings demonstrated the C.taliensis resources in the Lancang River Basin have moderate genetic diversity with moderate richness.This study offers a theoretical foundation for the establishment and preservation of C.taliensis germplasm resources as well as the creation of a genetic map.